Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis

Summary: Dysregulated angiogenesis signaling leads to pathological vascular growth and leakage, and is a hallmark of many diseases including cancer and ocular diseases. In peripheral arterial disease, the concomitant increase in vascular permeability presents significant challenges in therapeutic ef...

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Main Authors: Yu Zhang, Christopher D. Kontos, Brian H. Annex, Aleksander S. Popel
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589004225008867
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author Yu Zhang
Christopher D. Kontos
Brian H. Annex
Aleksander S. Popel
author_facet Yu Zhang
Christopher D. Kontos
Brian H. Annex
Aleksander S. Popel
author_sort Yu Zhang
collection DOAJ
description Summary: Dysregulated angiogenesis signaling leads to pathological vascular growth and leakage, and is a hallmark of many diseases including cancer and ocular diseases. In peripheral arterial disease, the concomitant increase in vascular permeability presents significant challenges in therapeutic efforts to improve perfusion by stimulating vascular growth. Building a mechanistic understanding of the endothelial control of vascular growth and permeability signaling is crucial to guide our efforts to identify therapeutic strategies that permit blood vessel growth while maintaining vascular stability. We develop a mechanistic systems biology model of the endothelial signaling network formed by the vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-Tie pathways, two major signaling pathways regulating vascular growth and stability. Our model, calibrated and validated against experimental data, reveals the mechanisms through which chronic Ang1 stimulation protects endothelial cells from VEGF-induced hyperpermeability, and predicts that combining Src inhibition with Tie2 activation can inhibit vascular leakage without disturbing angiogenesis signaling.
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publishDate 2025-06-01
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spelling doaj-art-90ecd8a0b7994e0fb61f80fdfa3adfec2025-08-20T03:13:03ZengElsevieriScience2589-00422025-06-0128611262510.1016/j.isci.2025.112625Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysisYu Zhang0Christopher D. Kontos1Brian H. Annex2Aleksander S. Popel3Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21215, USA; Corresponding authorDepartment of Medicine, Duke University Medical Center, Durham, NC 27710, USADepartment of Medicine, Augusta University, Augusta, GA 30912, USADepartment of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21215, USASummary: Dysregulated angiogenesis signaling leads to pathological vascular growth and leakage, and is a hallmark of many diseases including cancer and ocular diseases. In peripheral arterial disease, the concomitant increase in vascular permeability presents significant challenges in therapeutic efforts to improve perfusion by stimulating vascular growth. Building a mechanistic understanding of the endothelial control of vascular growth and permeability signaling is crucial to guide our efforts to identify therapeutic strategies that permit blood vessel growth while maintaining vascular stability. We develop a mechanistic systems biology model of the endothelial signaling network formed by the vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-Tie pathways, two major signaling pathways regulating vascular growth and stability. Our model, calibrated and validated against experimental data, reveals the mechanisms through which chronic Ang1 stimulation protects endothelial cells from VEGF-induced hyperpermeability, and predicts that combining Src inhibition with Tie2 activation can inhibit vascular leakage without disturbing angiogenesis signaling.http://www.sciencedirect.com/science/article/pii/S2589004225008867Natural sciencesBiological sciencesBioinformaticsPharmacoinformatics
spellingShingle Yu Zhang
Christopher D. Kontos
Brian H. Annex
Aleksander S. Popel
Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
iScience
Natural sciences
Biological sciences
Bioinformatics
Pharmacoinformatics
title Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
title_full Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
title_fullStr Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
title_full_unstemmed Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
title_short Promoting vascular stability through Src inhibition and Tie2 activation: A model-based analysis
title_sort promoting vascular stability through src inhibition and tie2 activation a model based analysis
topic Natural sciences
Biological sciences
Bioinformatics
Pharmacoinformatics
url http://www.sciencedirect.com/science/article/pii/S2589004225008867
work_keys_str_mv AT yuzhang promotingvascularstabilitythroughsrcinhibitionandtie2activationamodelbasedanalysis
AT christopherdkontos promotingvascularstabilitythroughsrcinhibitionandtie2activationamodelbasedanalysis
AT brianhannex promotingvascularstabilitythroughsrcinhibitionandtie2activationamodelbasedanalysis
AT aleksanderspopel promotingvascularstabilitythroughsrcinhibitionandtie2activationamodelbasedanalysis