Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method
ABSTRACT: The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is still lacking. In this study, BSA-galangin nanoparticles (BSA-GA NP) were designed for alleviating reactive oxygen species (ROS)–mediat...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224012098 |
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| author | Weigang Zhong Lei Xu Qi Wang Xue Shen |
| author_facet | Weigang Zhong Lei Xu Qi Wang Xue Shen |
| author_sort | Weigang Zhong |
| collection | DOAJ |
| description | ABSTRACT: The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is still lacking. In this study, BSA-galangin nanoparticles (BSA-GA NP) were designed for alleviating reactive oxygen species (ROS)–mediated macrophage inflammation by the 2 separate methods. Compared with the desolvation method, BSA exhibited a higher loading capacity for GA under the pH-shifting method, which was attributed to the exposure of the binding site leading to enhanced affinity for GA and a more compact particle structure. Further analyses evidenced that the electron arrangement and crystal structure of GA changed with different methods. The content of the random coil of BSA was elevated after the pH-shifting method. Additionally, the smaller size rendered the pH-shifting treated BSA-GA NP easier to be taken up by macrophages, and the enhanced specific surface area conferred excellent ROS scavenging and anti-inflammatory performances. This study may provide new insights into the choice of loading methods. |
| format | Article |
| id | doaj-art-90ea87d6a9fe4f269d7e05ee9b96e001 |
| institution | OA Journals |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-90ea87d6a9fe4f269d7e05ee9b96e0012025-08-20T01:57:20ZengElsevierJournal of Dairy Science0022-03022025-01-01108128229710.3168/jds.2024-25495Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting methodWeigang Zhong0Lei Xu1Qi Wang2Xue Shen3Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin, 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130012, ChinaDepartment of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin, 130062, ChinaDepartment of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin, 130062, China; Corresponding authorABSTRACT: The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is still lacking. In this study, BSA-galangin nanoparticles (BSA-GA NP) were designed for alleviating reactive oxygen species (ROS)–mediated macrophage inflammation by the 2 separate methods. Compared with the desolvation method, BSA exhibited a higher loading capacity for GA under the pH-shifting method, which was attributed to the exposure of the binding site leading to enhanced affinity for GA and a more compact particle structure. Further analyses evidenced that the electron arrangement and crystal structure of GA changed with different methods. The content of the random coil of BSA was elevated after the pH-shifting method. Additionally, the smaller size rendered the pH-shifting treated BSA-GA NP easier to be taken up by macrophages, and the enhanced specific surface area conferred excellent ROS scavenging and anti-inflammatory performances. This study may provide new insights into the choice of loading methods.http://www.sciencedirect.com/science/article/pii/S0022030224012098bovine serum albumingalanginethanol desolvationpH-shiftingnanoparticles |
| spellingShingle | Weigang Zhong Lei Xu Qi Wang Xue Shen Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method Journal of Dairy Science bovine serum albumin galangin ethanol desolvation pH-shifting nanoparticles |
| title | Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method |
| title_full | Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method |
| title_fullStr | Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method |
| title_full_unstemmed | Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method |
| title_short | Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method |
| title_sort | formation of bovine serum albumin galangin nanoparticles and their potential to inhibit reactive oxygen species induced inflammation ethanol desolvation versus ph shifting method |
| topic | bovine serum albumin galangin ethanol desolvation pH-shifting nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224012098 |
| work_keys_str_mv | AT weigangzhong formationofbovineserumalbumingalanginnanoparticlesandtheirpotentialtoinhibitreactiveoxygenspeciesinducedinflammationethanoldesolvationversusphshiftingmethod AT leixu formationofbovineserumalbumingalanginnanoparticlesandtheirpotentialtoinhibitreactiveoxygenspeciesinducedinflammationethanoldesolvationversusphshiftingmethod AT qiwang formationofbovineserumalbumingalanginnanoparticlesandtheirpotentialtoinhibitreactiveoxygenspeciesinducedinflammationethanoldesolvationversusphshiftingmethod AT xueshen formationofbovineserumalbumingalanginnanoparticlesandtheirpotentialtoinhibitreactiveoxygenspeciesinducedinflammationethanoldesolvationversusphshiftingmethod |