Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution
Abstract Tumor heterogeneity and tumor evolution contribute to cancer treatment failure. To understand how selective pressures drive heterogeneous tumor evolution, it would be useful to image multiple important components and tumor subclones in vivo. We propose a supercontinuum-tailoring two-photon...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-53697-1 |
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| author | Xiujuan Gao Xinyuan Huang Zhongyun Chen Liu Yang Yifu Zhou Zhenxuan Hou Jie Yang Shuhong Qi Zheng Liu Zhihong Zhang Qian Liu Qingming Luo Ling Fu |
| author_facet | Xiujuan Gao Xinyuan Huang Zhongyun Chen Liu Yang Yifu Zhou Zhenxuan Hou Jie Yang Shuhong Qi Zheng Liu Zhihong Zhang Qian Liu Qingming Luo Ling Fu |
| author_sort | Xiujuan Gao |
| collection | DOAJ |
| description | Abstract Tumor heterogeneity and tumor evolution contribute to cancer treatment failure. To understand how selective pressures drive heterogeneous tumor evolution, it would be useful to image multiple important components and tumor subclones in vivo. We propose a supercontinuum-tailoring two-photon microscope (SCT-TPM) and realize simultaneous observation of nine fluorophores with a single light beam, breaking through the ‘color barrier’ of intravital two-photon fluorescence imaging. It achieves excitation multiplexing only by modulating the phase of fiber supercontinuum (SC), allowing to capture rapid events of multiple targets with maintaining precise spatial alignment. We employ SCT-TPM to visualize the spatiotemporal dynamics of heterogeneous tumor evolution under host immune surveillance, particularly the behaviors and interactions of six tumor subclones, immune cells and vascular network, and thus infer the trajectories of tumor progression and clonal competition. SCT-TPM opens up the possibility of tumor lineage tracking and mechanism exploration in living biological systems. |
| format | Article |
| id | doaj-art-90d63323d5324cf5bdef49cb69be5a17 |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-90d63323d5324cf5bdef49cb69be5a172025-08-20T02:18:35ZengNature PortfolioNature Communications2041-17232024-10-0115111410.1038/s41467-024-53697-1Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolutionXiujuan Gao0Xinyuan Huang1Zhongyun Chen2Liu Yang3Yifu Zhou4Zhenxuan Hou5Jie Yang6Shuhong Qi7Zheng Liu8Zhihong Zhang9Qian Liu10Qingming Luo11Ling Fu12Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologySchool of Biomedical Engineering, Hainan UniversityBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologySchool of Biomedical Engineering, Hainan UniversitySchool of Biomedical Engineering, Hainan UniversityBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyAbstract Tumor heterogeneity and tumor evolution contribute to cancer treatment failure. To understand how selective pressures drive heterogeneous tumor evolution, it would be useful to image multiple important components and tumor subclones in vivo. We propose a supercontinuum-tailoring two-photon microscope (SCT-TPM) and realize simultaneous observation of nine fluorophores with a single light beam, breaking through the ‘color barrier’ of intravital two-photon fluorescence imaging. It achieves excitation multiplexing only by modulating the phase of fiber supercontinuum (SC), allowing to capture rapid events of multiple targets with maintaining precise spatial alignment. We employ SCT-TPM to visualize the spatiotemporal dynamics of heterogeneous tumor evolution under host immune surveillance, particularly the behaviors and interactions of six tumor subclones, immune cells and vascular network, and thus infer the trajectories of tumor progression and clonal competition. SCT-TPM opens up the possibility of tumor lineage tracking and mechanism exploration in living biological systems.https://doi.org/10.1038/s41467-024-53697-1 |
| spellingShingle | Xiujuan Gao Xinyuan Huang Zhongyun Chen Liu Yang Yifu Zhou Zhenxuan Hou Jie Yang Shuhong Qi Zheng Liu Zhihong Zhang Qian Liu Qingming Luo Ling Fu Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution Nature Communications |
| title | Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| title_full | Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| title_fullStr | Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| title_full_unstemmed | Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| title_short | Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| title_sort | supercontinuum tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution |
| url | https://doi.org/10.1038/s41467-024-53697-1 |
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