Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics
The application of aerobic denitrifying bacteria in biological nitrogen removal has been of great interest. However, the mechanism of nitrogen conversion by aerobic denitrifying bacteria is unclear due to the lack of stoichiometry and kinetic studies. Therefore, this study aimed to investigate the s...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2402445 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106101684502528 |
|---|---|
| author | Tian Hu Jiating Wei Yihang Ding Shuo Li Weicheng Zhang Jun Zhang |
| author_facet | Tian Hu Jiating Wei Yihang Ding Shuo Li Weicheng Zhang Jun Zhang |
| author_sort | Tian Hu |
| collection | DOAJ |
| description | The application of aerobic denitrifying bacteria in biological nitrogen removal has been of great interest. However, the mechanism of nitrogen conversion by aerobic denitrifying bacteria is unclear due to the lack of stoichiometry and kinetic studies. Therefore, this study aimed to investigate the stoichiometry and kinetics of aerobic denitrification using Pseudomonas mosselii H6 as a model strain. The results showed that the stoichiometric equations of strain H6 under different nitrogen source conditions were obtained by nitrogen balance analysis; the concentrations of different limiting substrates were set and combined with the multi-substrate Monod kinetic equation to fit the half-saturation constants of COD, ammonia, nitrate and nitrite nitrogen for strain H6, which were 230.1008, 25.3435, 4.1009 and 23.4601 mg/L, respectively; based on the model of “two-step denitrification”, the numerical simulation of the growth and aerobic denitrification of strain H6 under different limiting substrate concentrations was carried out by using AQUASIM software to test the validity of stoichiometric coefficients and kinetic constants and perfect fitting results were obtained. These parameters will help to simulate the growth and denitrification performance of aerobic denitrifying bacteria and provide an experimental basis for further quantitatively elucidating the mechanism of action in the aerobic denitrification process. |
| format | Article |
| id | doaj-art-90d22a6c5c5848e79fca90dca01d09f2 |
| institution | OA Journals |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-90d22a6c5c5848e79fca90dca01d09f22025-08-20T02:38:55ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2402445Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kineticsTian Hu0Jiating Wei1Yihang Ding2Shuo Li3Weicheng Zhang4Jun Zhang5College of Life Sciences, Nanyang Normal University, Nanyang, P. R. ChinaCollege of Life Sciences, Nanyang Normal University, Nanyang, P. R. ChinaCollege of Water Resources and Modern Agriculture, Nanyang Normal University, Nanyang, P. R. ChinaCollege of Water Resources and Modern Agriculture, Nanyang Normal University, Nanyang, P. R. ChinaCenter for Environment and Health in Water Source Area of South-to-North Water Diversion, School of Public Health, Hubei University of Medicine, Shiyan, ChinaCollaborative Innovation Center of Water Security for the Water Source Region of Mid-line of the South-to-North Diversion Project of Henan Province, Nanyang Normal University, Nanyang, P. R. ChinaThe application of aerobic denitrifying bacteria in biological nitrogen removal has been of great interest. However, the mechanism of nitrogen conversion by aerobic denitrifying bacteria is unclear due to the lack of stoichiometry and kinetic studies. Therefore, this study aimed to investigate the stoichiometry and kinetics of aerobic denitrification using Pseudomonas mosselii H6 as a model strain. The results showed that the stoichiometric equations of strain H6 under different nitrogen source conditions were obtained by nitrogen balance analysis; the concentrations of different limiting substrates were set and combined with the multi-substrate Monod kinetic equation to fit the half-saturation constants of COD, ammonia, nitrate and nitrite nitrogen for strain H6, which were 230.1008, 25.3435, 4.1009 and 23.4601 mg/L, respectively; based on the model of “two-step denitrification”, the numerical simulation of the growth and aerobic denitrification of strain H6 under different limiting substrate concentrations was carried out by using AQUASIM software to test the validity of stoichiometric coefficients and kinetic constants and perfect fitting results were obtained. These parameters will help to simulate the growth and denitrification performance of aerobic denitrifying bacteria and provide an experimental basis for further quantitatively elucidating the mechanism of action in the aerobic denitrification process.https://www.tandfonline.com/doi/10.1080/13102818.2024.2402445Aerobic denitrificationPseudomonas mosselii H6stoichiometric kineticstwo-step denitrification modeldenitrification mechanism |
| spellingShingle | Tian Hu Jiating Wei Yihang Ding Shuo Li Weicheng Zhang Jun Zhang Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics Biotechnology & Biotechnological Equipment Aerobic denitrification Pseudomonas mosselii H6 stoichiometric kinetics two-step denitrification model denitrification mechanism |
| title | Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics |
| title_full | Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics |
| title_fullStr | Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics |
| title_full_unstemmed | Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics |
| title_short | Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics |
| title_sort | characterisation of pseudomonas mosselii h6 for aerobic denitrification stoichiometry and reaction kinetics |
| topic | Aerobic denitrification Pseudomonas mosselii H6 stoichiometric kinetics two-step denitrification model denitrification mechanism |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2402445 |
| work_keys_str_mv | AT tianhu characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics AT jiatingwei characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics AT yihangding characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics AT shuoli characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics AT weichengzhang characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics AT junzhang characterisationofpseudomonasmosseliih6foraerobicdenitrificationstoichiometryandreactionkinetics |