Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils
The climate-driven acceleration of forest disease outbreaks has intensified the demand for sustainable biocontrol strategies. In this study, we evaluated the effects of the endophytic bacterium <i>Bacillus amyloliquefaciens</i> csuftcsp75 on soil properties, microbial communities, and fu...
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MDPI AG
2025-06-01
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| author | Quan Yang Shimeng Tan Anqi Niu Junang Liu Guoying Zhou |
| author_facet | Quan Yang Shimeng Tan Anqi Niu Junang Liu Guoying Zhou |
| author_sort | Quan Yang |
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| description | The climate-driven acceleration of forest disease outbreaks has intensified the demand for sustainable biocontrol strategies. In this study, we evaluated the effects of the endophytic bacterium <i>Bacillus amyloliquefaciens</i> csuftcsp75 on soil properties, microbial communities, and functional metabolism in soils affected by <i>Pinus massoniana</i> shoot blight. Soil physicochemical analysis, carbon substrate utilization profiling (AWCD), and diversity indices (the Shannon, Simpson, and McIntosh indices) were integrated to assess the microbial responses under different inoculation treatments. The csuftcsp75 treatment significantly improved soil nutrient availability—especially available phosphorus and potassium—and was associated with enhanced microbial metabolic activity and sustained functional diversity. Principal component analysis and correlation mapping revealed strong associations between labile nutrients and microbial responses. Comparative analysis showed that csuftcsp75 promoted a balanced and metabolically rich microbial community, while less compatible strains exhibited transient or unstable effects. These findings support a dual-pathway model wherein nutrient-driven metabolic activation and ecological integration jointly determine biocontrol efficacy. This study highlights the importance of matching microbial inoculants with local soil environments to optimize functional outcomes. This work provides a theoretical basis for applying endophytic <i>Bacillus</i> in forest disease management and contributes to the development of ecologically coherent biocontrol strategies. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-2607 |
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| series | Microorganisms |
| spelling | doaj-art-90d181ec4aee4b0998c21bd82344b63d2025-08-20T02:47:14ZengMDPI AGMicroorganisms2076-26072025-06-01137145410.3390/microorganisms13071454Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest SoilsQuan Yang0Shimeng Tan1Anqi Niu2Junang Liu3Guoying Zhou4Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou 571100, ChinaKey Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaThe climate-driven acceleration of forest disease outbreaks has intensified the demand for sustainable biocontrol strategies. In this study, we evaluated the effects of the endophytic bacterium <i>Bacillus amyloliquefaciens</i> csuftcsp75 on soil properties, microbial communities, and functional metabolism in soils affected by <i>Pinus massoniana</i> shoot blight. Soil physicochemical analysis, carbon substrate utilization profiling (AWCD), and diversity indices (the Shannon, Simpson, and McIntosh indices) were integrated to assess the microbial responses under different inoculation treatments. The csuftcsp75 treatment significantly improved soil nutrient availability—especially available phosphorus and potassium—and was associated with enhanced microbial metabolic activity and sustained functional diversity. Principal component analysis and correlation mapping revealed strong associations between labile nutrients and microbial responses. Comparative analysis showed that csuftcsp75 promoted a balanced and metabolically rich microbial community, while less compatible strains exhibited transient or unstable effects. These findings support a dual-pathway model wherein nutrient-driven metabolic activation and ecological integration jointly determine biocontrol efficacy. This study highlights the importance of matching microbial inoculants with local soil environments to optimize functional outcomes. This work provides a theoretical basis for applying endophytic <i>Bacillus</i> in forest disease management and contributes to the development of ecologically coherent biocontrol strategies.https://www.mdpi.com/2076-2607/13/7/1454endophytic <i>Bacillus amyloliquefaciens</i><i>Pinus massoniana</i> shoot blightmicrobial functional diversitybiological controlforest soil health |
| spellingShingle | Quan Yang Shimeng Tan Anqi Niu Junang Liu Guoying Zhou Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils Microorganisms endophytic <i>Bacillus amyloliquefaciens</i> <i>Pinus massoniana</i> shoot blight microbial functional diversity biological control forest soil health |
| title | Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils |
| title_full | Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils |
| title_fullStr | Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils |
| title_full_unstemmed | Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils |
| title_short | Nutrient-Driven Metabolic Activation and Microbial Restructuring Induced by Endophytic <i>Bacillus</i> in Blight-Affected Forest Soils |
| title_sort | nutrient driven metabolic activation and microbial restructuring induced by endophytic i bacillus i in blight affected forest soils |
| topic | endophytic <i>Bacillus amyloliquefaciens</i> <i>Pinus massoniana</i> shoot blight microbial functional diversity biological control forest soil health |
| url | https://www.mdpi.com/2076-2607/13/7/1454 |
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