Prokaryotic expression of full coat protein gene of Tomato spotted wilt virus and development of Dot-blot ELISA method for this virus detection
The full coat protein gene of Tomato spotted wilt virus (TSWV) was subcloned into a prokaryotic expression vector pET-32a. Results of restriction enzyme digestion and DNA sequencing show that the insertion direction and sequence were correct and no frameshift mutation existed. This recombinant proka...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.06.002 |
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