Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling
Abstract Marek’s Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F6 population from a full-sib adva...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-83356-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559449380061184 |
|---|---|
| author | Ehud Lipkin Jacqueline Smith Morris Soller David W. Burt Janet E. Fulton |
| author_facet | Ehud Lipkin Jacqueline Smith Morris Soller David W. Burt Janet E. Fulton |
| author_sort | Ehud Lipkin |
| collection | DOAJ |
| description | Abstract Marek’s Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty-seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P and LD values were used to assess the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD. |
| format | Article |
| id | doaj-art-90c3ab5ce9b5469c98b591efb3afd301 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-90c3ab5ce9b5469c98b591efb3afd3012025-01-05T12:29:23ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-83356-wMapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA poolingEhud Lipkin0Jacqueline Smith1Morris Soller2David W. Burt3Janet E. Fulton4Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of JerusalemThe Roslin Institute and Royal (Dick) School of Veterinary Studies R(D)SVS, University of EdinburghDepartment of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of JerusalemThe Roslin Institute and Royal (Dick) School of Veterinary Studies R(D)SVS, University of EdinburghHy-Line InternationalAbstract Marek’s Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty-seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P and LD values were used to assess the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD.https://doi.org/10.1038/s41598-024-83356-wMarek’s DiseaseDisease resistanceQTLRLinkage disequilibriumAllele substitution effectChicken |
| spellingShingle | Ehud Lipkin Jacqueline Smith Morris Soller David W. Burt Janet E. Fulton Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling Scientific Reports Marek’s Disease Disease resistance QTLR Linkage disequilibrium Allele substitution effect Chicken |
| title | Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling |
| title_full | Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling |
| title_fullStr | Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling |
| title_full_unstemmed | Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling |
| title_short | Mapping quantitative trait loci regions associated with Marek’s disease on chicken autosomes by means of selective DNA pooling |
| title_sort | mapping quantitative trait loci regions associated with marek s disease on chicken autosomes by means of selective dna pooling |
| topic | Marek’s Disease Disease resistance QTLR Linkage disequilibrium Allele substitution effect Chicken |
| url | https://doi.org/10.1038/s41598-024-83356-w |
| work_keys_str_mv | AT ehudlipkin mappingquantitativetraitlociregionsassociatedwithmareksdiseaseonchickenautosomesbymeansofselectivednapooling AT jacquelinesmith mappingquantitativetraitlociregionsassociatedwithmareksdiseaseonchickenautosomesbymeansofselectivednapooling AT morrissoller mappingquantitativetraitlociregionsassociatedwithmareksdiseaseonchickenautosomesbymeansofselectivednapooling AT davidwburt mappingquantitativetraitlociregionsassociatedwithmareksdiseaseonchickenautosomesbymeansofselectivednapooling AT janetefulton mappingquantitativetraitlociregionsassociatedwithmareksdiseaseonchickenautosomesbymeansofselectivednapooling |