Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo
Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2015-0032 |
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| author | Croitoru Mircea Dumitru Petkes Hermina Iulia Fülöp Ibolya Cotârlan Remus Şerban Oana Elena Dogaru Titica Maria Gâz Florea Şerban Andrei Tőkés Béla Majdik Cornelia |
| author_facet | Croitoru Mircea Dumitru Petkes Hermina Iulia Fülöp Ibolya Cotârlan Remus Şerban Oana Elena Dogaru Titica Maria Gâz Florea Şerban Andrei Tőkés Béla Majdik Cornelia |
| author_sort | Croitoru Mircea Dumitru |
| collection | DOAJ |
| description | Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical. |
| format | Article |
| id | doaj-art-90c3453fda734a218f5dc90d3d9a95f4 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-90c3453fda734a218f5dc90d3d9a95f42025-02-02T15:47:00ZengSciendoActa Pharmaceutica1846-95582015-12-0165441342610.1515/acph-2015-0032acph-2015-0032Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivoCroitoru Mircea Dumitru0Petkes Hermina Iulia1Fülöp Ibolya2Cotârlan Remus3Şerban Oana Elena4Dogaru Titica Maria5Gâz Florea Şerban Andrei6Tőkés Béla7Majdik Cornelia8Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Chemistry and Chemical Engineering, Babes-Bolyai University Cluj-Napoca 400028, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş Tîrgu Mureş 540138, RomaniaFaculty of Chemistry and Chemical Engineering, Babes-Bolyai University Cluj-Napoca 400028, RomaniaFree radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.https://doi.org/10.1515/acph-2015-0032nitric oxidenitritenitratenitronesnitric oxide donor |
| spellingShingle | Croitoru Mircea Dumitru Petkes Hermina Iulia Fülöp Ibolya Cotârlan Remus Şerban Oana Elena Dogaru Titica Maria Gâz Florea Şerban Andrei Tőkés Béla Majdik Cornelia Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo Acta Pharmaceutica nitric oxide nitrite nitrate nitrones nitric oxide donor |
| title | Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo |
| title_full | Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo |
| title_fullStr | Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo |
| title_full_unstemmed | Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo |
| title_short | Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo |
| title_sort | nitrones not only extraordinary spin traps but also good nitric oxide sources in vivo |
| topic | nitric oxide nitrite nitrate nitrones nitric oxide donor |
| url | https://doi.org/10.1515/acph-2015-0032 |
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