Optimization and assessment of an integrated workflow for the isolation and proteomic analysis of small urinary extracellular vesicles (suEVs)

Optimization and assessment of an integrated workflow for the isolation and proteomic analysis of small urinary extracellular vesicles (suEVs)

Small urinary extracellular vesicles (suEVs) are 50–200 nm membrane-delimited vesicles secreted mainly by urothelial cells. suEVs have become a promising non-invasive source of biomarkers for urinary diseases. However, suEV proteomic studies are limited due to the low concentration of EVs in urine s...

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Bibliographic Details
Main Authors: Yilan Hu, Jifeng Wang, Xiaoqing Qing, Tanxi Cai, Lili Niu, Xiang Ding, Zhensheng Xie, Mengmeng Zhang, Xiaojing Guo, Xiulan Chen, Fuquan Yang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Extracellular Vesicle
Subjects:
Small urinary extracellular vesicles
Proteomics
Uromodulin
Ultracentrifugation
Membrane filtration
Online Access:http://www.sciencedirect.com/science/article/pii/S2773041725000125
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