Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut
IntroductionMSTN is a well-studied inhibitor of skeletal muscle development, but its mechanism of affecting gut metabolites and the functions it exerts through this pathway are still unclear. This study aims to reveal how MSTN affects the metabolism process by regulating gut metabolites.MethodsCombi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1541257/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850140499347767296 |
|---|---|
| author | Li Gao Yong Ma Lili Wang Hao Wu Ruobing Kang Guangpeng Li Lei Yang Tong Wen |
| author_facet | Li Gao Yong Ma Lili Wang Hao Wu Ruobing Kang Guangpeng Li Lei Yang Tong Wen |
| author_sort | Li Gao |
| collection | DOAJ |
| description | IntroductionMSTN is a well-studied inhibitor of skeletal muscle development, but its mechanism of affecting gut metabolites and the functions it exerts through this pathway are still unclear. This study aims to reveal how MSTN affects the metabolism process by regulating gut metabolites.MethodsCombined analysis of jejunal contents metabolome and jejunal tissue transcriptome was used to compare the differences in intestinal metabolites and intestinal tissue gene expression between MSTN mutant and wild-type bovines.ResultsMetabolomic analysis identified that compared to wild-type bovine, the abundance of 304 metabolites were significantly changed in MSTN mutant cattle including 142 upregulated and 162 downregulated. Transcriptome results showed that the expression level of 1541 genes were influenced by MSTN disruption, including 536 upregulated genes and 1005 downregulated genes, which were categorized into 311 KEGG signaling pathways, primarily related to disease and metabolism. Correlation analysis results suggested a notable cross-regulation between the transcript levels of some specific genes in jejunal tissues and the abundance of jejunal metabolites, represented by fatty metabolites and genes associated with fatty acid degradation, synthesis and elongation.DiscussionCollectively, the result of this study indicated that MSTN gene mutation led to alterations in gut microbial metabolites by increasing the abundance of beneficial monounsaturated fatty acids (MUFAs) such as oleic acid, then to promote fatty acid degradation while inhibiting its synthesis by regulating the expression levels of relevant genes. These results provide a foundation for understanding the effects of MSTN gene mutations on gut metabolites and its certain functions that MSTN regulated via gut metabolites |
| format | Article |
| id | doaj-art-90bdc1f3699b4a19953de501b6275da4 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-90bdc1f3699b4a19953de501b6275da42025-08-20T02:29:46ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.15412571541257Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gutLi Gao0Yong Ma1Lili Wang2Hao Wu3Ruobing Kang4Guangpeng Li5Lei Yang6Tong Wen7College of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaCollege of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaCollege of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaCollege of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaCollege of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaCollege of Ecology and Environment, Baotou Teacher’s College, Baotou, ChinaIntroductionMSTN is a well-studied inhibitor of skeletal muscle development, but its mechanism of affecting gut metabolites and the functions it exerts through this pathway are still unclear. This study aims to reveal how MSTN affects the metabolism process by regulating gut metabolites.MethodsCombined analysis of jejunal contents metabolome and jejunal tissue transcriptome was used to compare the differences in intestinal metabolites and intestinal tissue gene expression between MSTN mutant and wild-type bovines.ResultsMetabolomic analysis identified that compared to wild-type bovine, the abundance of 304 metabolites were significantly changed in MSTN mutant cattle including 142 upregulated and 162 downregulated. Transcriptome results showed that the expression level of 1541 genes were influenced by MSTN disruption, including 536 upregulated genes and 1005 downregulated genes, which were categorized into 311 KEGG signaling pathways, primarily related to disease and metabolism. Correlation analysis results suggested a notable cross-regulation between the transcript levels of some specific genes in jejunal tissues and the abundance of jejunal metabolites, represented by fatty metabolites and genes associated with fatty acid degradation, synthesis and elongation.DiscussionCollectively, the result of this study indicated that MSTN gene mutation led to alterations in gut microbial metabolites by increasing the abundance of beneficial monounsaturated fatty acids (MUFAs) such as oleic acid, then to promote fatty acid degradation while inhibiting its synthesis by regulating the expression levels of relevant genes. These results provide a foundation for understanding the effects of MSTN gene mutations on gut metabolites and its certain functions that MSTN regulated via gut metaboliteshttps://www.frontiersin.org/articles/10.3389/fvets.2025.1541257/fullMSTNbovinegutmetabolometranscriptome |
| spellingShingle | Li Gao Yong Ma Lili Wang Hao Wu Ruobing Kang Guangpeng Li Lei Yang Tong Wen Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut Frontiers in Veterinary Science MSTN bovine gut metabolome transcriptome |
| title | Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut |
| title_full | Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut |
| title_fullStr | Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut |
| title_full_unstemmed | Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut |
| title_short | Combined metabolome and transcriptome analysis revealed that MSTN regulated the process of bovine fatty acid metabolism in gut |
| title_sort | combined metabolome and transcriptome analysis revealed that mstn regulated the process of bovine fatty acid metabolism in gut |
| topic | MSTN bovine gut metabolome transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1541257/full |
| work_keys_str_mv | AT ligao combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT yongma combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT liliwang combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT haowu combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT ruobingkang combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT guangpengli combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT leiyang combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut AT tongwen combinedmetabolomeandtranscriptomeanalysisrevealedthatmstnregulatedtheprocessofbovinefattyacidmetabolismingut |