New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase
Diverse pretreatment methods have been developed for cell-cracking and sludge denaturation to harvest pretreated sludge as the suitable fermentation substrate, but new inoculation is not always working quickly or adaptively for the pretreated sludge. This study established a sludge SDMP method by in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Water Research X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589914725000222 |
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| author | Xiqi Li Aijie Wang Zhe Yu Hualin Wang Hengzhi You Jifei Xu Jingyu Zhang Wenzong Liu |
| author_facet | Xiqi Li Aijie Wang Zhe Yu Hualin Wang Hengzhi You Jifei Xu Jingyu Zhang Wenzong Liu |
| author_sort | Xiqi Li |
| collection | DOAJ |
| description | Diverse pretreatment methods have been developed for cell-cracking and sludge denaturation to harvest pretreated sludge as the suitable fermentation substrate, but new inoculation is not always working quickly or adaptively for the pretreated sludge. This study established a sludge SDMP method by integrating RL and HSHC to fully use the enriched microorganisms with decomposing enzymes/abilities at the sludge decline phase for organic matters bioconversion. The SDMP method cracked EPS but preserved and enhanced the microbial viability and the activity of enzymes related to hydrolysis and acidifying. Then the VFAs production cycle of SDMP sludge was shortened by 36.25 % than alkaline pretreated sludge, and the yield reached 4482.19 mg COD/L in 3 days with the organic matter conversion rate of 369.36 mg COD/g VSS. Preserved and enriched sludge self-degradation microorganisms composed a critical part of the dominant community, including Mycobacterium, norank_f__Pirellulaceae, IMCC26207, norank_f__JG30-KF-CM45, and Petrimonas. The interaction network centered on them facilitated acidogenic metabolism by reinforcing pathways such as glycolysis, pyruvate metabolism, and the Stickland reaction in amino acid metabolism. This study provides new insights into developing microbial functions for recycling resources from sludge. |
| format | Article |
| id | doaj-art-90a6a2d8d25d48b496d0f1b779a8fd6a |
| institution | OA Journals |
| issn | 2589-9147 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Water Research X |
| spelling | doaj-art-90a6a2d8d25d48b496d0f1b779a8fd6a2025-08-20T02:05:52ZengElsevierWater Research X2589-91472025-05-012710032310.1016/j.wroa.2025.100323New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phaseXiqi Li0Aijie Wang1Zhe Yu2Hualin Wang3Hengzhi You4Jifei Xu5Jingyu Zhang6Wenzong Liu7State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, PR China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150001, PR ChinaState Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, PR China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150001, PR China; CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150001, PR ChinaNational Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai, 200237, PR ChinaSchool of Biomedical Engineering and Digital Health, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, PR ChinaInner Mongolia Key Laboratory of Environmental Pollution Prevention and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010000, PR ChinaInner Mongolia Environmental Protection Investment Group Co. Ltd, Hohhot, 010000, PR ChinaState Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, PR China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150001, PR China; Corresponding author.Diverse pretreatment methods have been developed for cell-cracking and sludge denaturation to harvest pretreated sludge as the suitable fermentation substrate, but new inoculation is not always working quickly or adaptively for the pretreated sludge. This study established a sludge SDMP method by integrating RL and HSHC to fully use the enriched microorganisms with decomposing enzymes/abilities at the sludge decline phase for organic matters bioconversion. The SDMP method cracked EPS but preserved and enhanced the microbial viability and the activity of enzymes related to hydrolysis and acidifying. Then the VFAs production cycle of SDMP sludge was shortened by 36.25 % than alkaline pretreated sludge, and the yield reached 4482.19 mg COD/L in 3 days with the organic matter conversion rate of 369.36 mg COD/g VSS. Preserved and enriched sludge self-degradation microorganisms composed a critical part of the dominant community, including Mycobacterium, norank_f__Pirellulaceae, IMCC26207, norank_f__JG30-KF-CM45, and Petrimonas. The interaction network centered on them facilitated acidogenic metabolism by reinforcing pathways such as glycolysis, pyruvate metabolism, and the Stickland reaction in amino acid metabolism. This study provides new insights into developing microbial functions for recycling resources from sludge.http://www.sciencedirect.com/science/article/pii/S2589914725000222Waste-activated sludgeMicrobial growth cycleExtracellular polymeric substancesSludge self-degradation microorganismsVolatile fatty acids |
| spellingShingle | Xiqi Li Aijie Wang Zhe Yu Hualin Wang Hengzhi You Jifei Xu Jingyu Zhang Wenzong Liu New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase Water Research X Waste-activated sludge Microbial growth cycle Extracellular polymeric substances Sludge self-degradation microorganisms Volatile fatty acids |
| title | New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase |
| title_full | New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase |
| title_fullStr | New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase |
| title_full_unstemmed | New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase |
| title_short | New understanding of microbial growth cycle for efficient waste-activated sludge disposal by preserving microbial self-degradation activity during the decline phase |
| title_sort | new understanding of microbial growth cycle for efficient waste activated sludge disposal by preserving microbial self degradation activity during the decline phase |
| topic | Waste-activated sludge Microbial growth cycle Extracellular polymeric substances Sludge self-degradation microorganisms Volatile fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S2589914725000222 |
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