Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii.
Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a se...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0069532&type=printable |
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| author | Michelle M Leger Ryan M R Gawryluk Michael W Gray Andrew J Roger |
| author_facet | Michelle M Leger Ryan M R Gawryluk Michael W Gray Andrew J Roger |
| author_sort | Michelle M Leger |
| collection | DOAJ |
| description | Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes. |
| format | Article |
| id | doaj-art-90a1e2b8307542bfa7b6d6389b945b96 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-90a1e2b8307542bfa7b6d6389b945b962025-08-20T03:47:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e6953210.1371/journal.pone.0069532Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii.Michelle M LegerRyan M R GawrylukMichael W GrayAndrew J RogerDiverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0069532&type=printable |
| spellingShingle | Michelle M Leger Ryan M R Gawryluk Michael W Gray Andrew J Roger Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. PLoS ONE |
| title | Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. |
| title_full | Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. |
| title_fullStr | Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. |
| title_full_unstemmed | Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. |
| title_short | Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii. |
| title_sort | evidence for a hydrogenosomal type anaerobic atp generation pathway in acanthamoeba castellanii |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0069532&type=printable |
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