Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery
The <i>Aspergillus</i> genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from <i>Aspergillus</i> spp., their metabolic potent...
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MDPI AG
2025-06-01
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| author | Yujie Zhao Qing Gong Huawei Zhang |
| author_facet | Yujie Zhao Qing Gong Huawei Zhang |
| author_sort | Yujie Zhao |
| collection | DOAJ |
| description | The <i>Aspergillus</i> genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from <i>Aspergillus</i> spp., their metabolic potential remains unexplored due to the presence of numerous silent biosynthetic gene clusters (BGCs) in their genomes. Fortunately, over the last two decades, the global transcriptional regulator (GTR) engineering strategy has emerged as a powerful tool for activating these cryptic BGCs in order to synthesize previously undiscovered SMs from <i>Aspergillus</i> spp. This review highlights recent advances in fungal GTR engineering techniques, the regulatory mechanisms of GTRs, and current challenges and future perspectives for their application in natural product discovery in the genus <i>Aspergillus</i>. |
| format | Article |
| id | doaj-art-909cd60b128c4dada750f9adca574c4e |
| institution | OA Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-909cd60b128c4dada750f9adca574c4e2025-08-20T02:21:03ZengMDPI AGJournal of Fungi2309-608X2025-06-0111644910.3390/jof11060449Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product DiscoveryYujie Zhao0Qing Gong1Huawei Zhang2School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaThe <i>Aspergillus</i> genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from <i>Aspergillus</i> spp., their metabolic potential remains unexplored due to the presence of numerous silent biosynthetic gene clusters (BGCs) in their genomes. Fortunately, over the last two decades, the global transcriptional regulator (GTR) engineering strategy has emerged as a powerful tool for activating these cryptic BGCs in order to synthesize previously undiscovered SMs from <i>Aspergillus</i> spp. This review highlights recent advances in fungal GTR engineering techniques, the regulatory mechanisms of GTRs, and current challenges and future perspectives for their application in natural product discovery in the genus <i>Aspergillus</i>.https://www.mdpi.com/2309-608X/11/6/449global transcriptional regulatorssecondary metabolitesregulatory mechanismsCRISPR/Cas9homologous recombination |
| spellingShingle | Yujie Zhao Qing Gong Huawei Zhang Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery Journal of Fungi global transcriptional regulators secondary metabolites regulatory mechanisms CRISPR/Cas9 homologous recombination |
| title | Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery |
| title_full | Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery |
| title_fullStr | Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery |
| title_full_unstemmed | Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery |
| title_short | Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery |
| title_sort | engineering of global transcriptional regulators gtrs in i aspergillus i for natural product discovery |
| topic | global transcriptional regulators secondary metabolites regulatory mechanisms CRISPR/Cas9 homologous recombination |
| url | https://www.mdpi.com/2309-608X/11/6/449 |
| work_keys_str_mv | AT yujiezhao engineeringofglobaltranscriptionalregulatorsgtrsiniaspergillusifornaturalproductdiscovery AT qinggong engineeringofglobaltranscriptionalregulatorsgtrsiniaspergillusifornaturalproductdiscovery AT huaweizhang engineeringofglobaltranscriptionalregulatorsgtrsiniaspergillusifornaturalproductdiscovery |