Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System
Molecular imaging holds great promise in the noninvasive monitoring of several diseases with nanoparticles (NPs) being considered an efficient imaging tool for cancer, central nervous system, and heart- or bone-related diseases and for disorders of the mononuclear phagocytic system (MPS). In the pre...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-01-01
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| Series: | Molecular Imaging |
| Online Access: | http://dx.doi.org/10.1155/2021/6677847 |
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| author | Maritina Rouchota Alessio Adamiano Michele Iafisco Eirini Fragogeorgi Irineos Pilatis Gilles Doumont Sébastien Boutry Daniele Catalucci Argyro Zacharioudaki George C. Kagadis |
| author_facet | Maritina Rouchota Alessio Adamiano Michele Iafisco Eirini Fragogeorgi Irineos Pilatis Gilles Doumont Sébastien Boutry Daniele Catalucci Argyro Zacharioudaki George C. Kagadis |
| author_sort | Maritina Rouchota |
| collection | DOAJ |
| description | Molecular imaging holds great promise in the noninvasive monitoring of several diseases with nanoparticles (NPs) being considered an efficient imaging tool for cancer, central nervous system, and heart- or bone-related diseases and for disorders of the mononuclear phagocytic system (MPS). In the present study, we used an iron-based nanoformulation, already established as an MRI/SPECT probe, as well as to load different biomolecules, to investigate its potential for nuclear planar and tomographic imaging of several target tissues following its distribution via different administration routes. Iron-doped hydroxyapatite NPs (FeHA) were radiolabeled with the single photon γ-emitting imaging agent [99mTc]TcMDP. Administration of the radioactive NPs was performed via the following four delivery methods: (1) standard intravenous (iv) tail vein, (2) iv retro-orbital injection, (3) intratracheal (it) instillation, and (4) intrarectal installation (pr). Real-time, live, fast dynamic screening studies were performed on a dedicated bench top, mouse-sized, planar SPECT system from t=0 to 1 hour postinjection (p.i.), and consequently, tomographic SPECT/CT imaging was performed, for up to 24 hours p.i. The administration routes that have been studied provide a wide range of possible target tissues, for various diseases. Studies can be optimized following this workflow, as it is possible to quickly assess more parameters in a small number of animals (injection route, dosage, and fasting conditions). Thus, such an imaging protocol combines the strengths of both dynamic planar and tomographic imaging, and by using iron-based NPs of high biocompatibility along with the appropriate administration route, a potential diagnostic or therapeutic effect could be attained. |
| format | Article |
| id | doaj-art-909b2119d71241159cbfe4149787db6d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-909b2119d71241159cbfe4149787db6d2025-02-03T10:12:45ZengSAGE PublishingMolecular Imaging1536-01212021-01-01202110.1155/2021/66778476677847Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles SystemMaritina Rouchota0Alessio Adamiano1Michele Iafisco2Eirini Fragogeorgi3Irineos Pilatis4Gilles Doumont5Sébastien Boutry6Daniele Catalucci7Argyro Zacharioudaki8George C. Kagadis93dmi Research Group, Department of Medical Physics, School of Medicine, University of Patras, GreeceInstitute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), ItalyInstitute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), ItalyInstitute of Nuclear & Radiological Sciences, Technology, Energy & Safety, NCSR “Demokritos”, GreeceDepartment of Biomedical Engineering, University of West Attica, GreeceCenter for Microscopy and Molecular Imaging (CMMI), Université Libre de Bruxelles (ULB), Rue Adrienne Bolland 8, B-6041 Charleroi (Gosselies), BelgiumCenter for Microscopy and Molecular Imaging (CMMI), Université Libre de Bruxelles (ULB), Rue Adrienne Bolland 8, B-6041 Charleroi (Gosselies), BelgiumInstitute of Genetic and Biomedical Research (IRGB), National Research Council (CNR), UOS Milan, ItalyDVM MLAS Dipl.ECLAM, Greece3dmi Research Group, Department of Medical Physics, School of Medicine, University of Patras, GreeceMolecular imaging holds great promise in the noninvasive monitoring of several diseases with nanoparticles (NPs) being considered an efficient imaging tool for cancer, central nervous system, and heart- or bone-related diseases and for disorders of the mononuclear phagocytic system (MPS). In the present study, we used an iron-based nanoformulation, already established as an MRI/SPECT probe, as well as to load different biomolecules, to investigate its potential for nuclear planar and tomographic imaging of several target tissues following its distribution via different administration routes. Iron-doped hydroxyapatite NPs (FeHA) were radiolabeled with the single photon γ-emitting imaging agent [99mTc]TcMDP. Administration of the radioactive NPs was performed via the following four delivery methods: (1) standard intravenous (iv) tail vein, (2) iv retro-orbital injection, (3) intratracheal (it) instillation, and (4) intrarectal installation (pr). Real-time, live, fast dynamic screening studies were performed on a dedicated bench top, mouse-sized, planar SPECT system from t=0 to 1 hour postinjection (p.i.), and consequently, tomographic SPECT/CT imaging was performed, for up to 24 hours p.i. The administration routes that have been studied provide a wide range of possible target tissues, for various diseases. Studies can be optimized following this workflow, as it is possible to quickly assess more parameters in a small number of animals (injection route, dosage, and fasting conditions). Thus, such an imaging protocol combines the strengths of both dynamic planar and tomographic imaging, and by using iron-based NPs of high biocompatibility along with the appropriate administration route, a potential diagnostic or therapeutic effect could be attained.http://dx.doi.org/10.1155/2021/6677847 |
| spellingShingle | Maritina Rouchota Alessio Adamiano Michele Iafisco Eirini Fragogeorgi Irineos Pilatis Gilles Doumont Sébastien Boutry Daniele Catalucci Argyro Zacharioudaki George C. Kagadis Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System Molecular Imaging |
| title | Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System |
| title_full | Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System |
| title_fullStr | Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System |
| title_full_unstemmed | Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System |
| title_short | Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System |
| title_sort | optimization of in vivo studies by combining planar dynamic and tomographic imaging workflow evaluation on a superparamagnetic nanoparticles system |
| url | http://dx.doi.org/10.1155/2021/6677847 |
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