Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments
This study addresses the challenge of finding novel ways to solubilize phosphorus and zinc for agricultural purposes. The aim was to isolate PSMs (phosphorous-solubilizing microbes) and ZnSMs (zinc-solubilizing microbes) from different environments (e.g., soil amendments, land uses, and crop rotatio...
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MDPI AG
2024-07-01
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| author | Samira Islas-Valdez Antisar Afkairin Benjamin Rovner Jorge M. Vivanco |
| author_facet | Samira Islas-Valdez Antisar Afkairin Benjamin Rovner Jorge M. Vivanco |
| author_sort | Samira Islas-Valdez |
| collection | DOAJ |
| description | This study addresses the challenge of finding novel ways to solubilize phosphorus and zinc for agricultural purposes. The aim was to isolate PSMs (phosphorous-solubilizing microbes) and ZnSMs (zinc-solubilizing microbes) from different environments (e.g., soil amendments, land uses, and crop rotation systems) and evaluate their ability to solubilize different insoluble P sources (e.g., β-tricalcium phosphate (β-TCP), calcium-phytate (CaP), and rock phosphate (RP)) and Zn sources (e.g., zinc carbonate (ZnC), zinc oxide (ZnO), and zinc phosphate (ZnP)). Here, 25 isolates capable of solubilizing either P or Zn sources were isolated and classified by species using 16S rRNA and ITS-region sequencing. Notably, <i>Aspergillus awamori</i>, <i>Fusarium circinatum</i>, <i>Fusarium longifundum</i>, and <i>Mucor circinelloides</i>, isolated from cultivated soils and soil amendments, emerged as the most efficient PSMs and ZnSMs. <i>Mucor circinelloides</i> exhibited the highest solubilization ability for broths containing β-TCP, CaP, RP, ZnO, and ZnP, with log2-fold changes of 3.7, 1.8, 8.9, 7.8, and 2.4, respectively, compared to the control. For ZnC and ZnO, <i>Aspergillus awamori</i> displayed the highest Zn solubilization, with a 2.1 and 3.0 log2-fold change. The study highlights the potential of these strains as biofertilizers and underscores the role of <i>Mucor</i> and <i>Fusarium</i> genera in zinc solubilization. |
| format | Article |
| id | doaj-art-908ada83c4ed4ae6b5aa29db987e451e |
| institution | OA Journals |
| issn | 2673-8007 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj-art-908ada83c4ed4ae6b5aa29db987e451e2025-08-20T01:55:58ZengMDPI AGApplied Microbiology2673-80072024-07-01431042105610.3390/applmicrobiol4030071Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different EnvironmentsSamira Islas-Valdez0Antisar Afkairin1Benjamin Rovner2Jorge M. Vivanco3Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USADepartment of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USADepartment of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USADepartment of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USAThis study addresses the challenge of finding novel ways to solubilize phosphorus and zinc for agricultural purposes. The aim was to isolate PSMs (phosphorous-solubilizing microbes) and ZnSMs (zinc-solubilizing microbes) from different environments (e.g., soil amendments, land uses, and crop rotation systems) and evaluate their ability to solubilize different insoluble P sources (e.g., β-tricalcium phosphate (β-TCP), calcium-phytate (CaP), and rock phosphate (RP)) and Zn sources (e.g., zinc carbonate (ZnC), zinc oxide (ZnO), and zinc phosphate (ZnP)). Here, 25 isolates capable of solubilizing either P or Zn sources were isolated and classified by species using 16S rRNA and ITS-region sequencing. Notably, <i>Aspergillus awamori</i>, <i>Fusarium circinatum</i>, <i>Fusarium longifundum</i>, and <i>Mucor circinelloides</i>, isolated from cultivated soils and soil amendments, emerged as the most efficient PSMs and ZnSMs. <i>Mucor circinelloides</i> exhibited the highest solubilization ability for broths containing β-TCP, CaP, RP, ZnO, and ZnP, with log2-fold changes of 3.7, 1.8, 8.9, 7.8, and 2.4, respectively, compared to the control. For ZnC and ZnO, <i>Aspergillus awamori</i> displayed the highest Zn solubilization, with a 2.1 and 3.0 log2-fold change. The study highlights the potential of these strains as biofertilizers and underscores the role of <i>Mucor</i> and <i>Fusarium</i> genera in zinc solubilization.https://www.mdpi.com/2673-8007/4/3/71P-solubilizing microorganismsZn-solubilizing microorganismssoil amendmentscultivated soilsbacteriafungi |
| spellingShingle | Samira Islas-Valdez Antisar Afkairin Benjamin Rovner Jorge M. Vivanco Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments Applied Microbiology P-solubilizing microorganisms Zn-solubilizing microorganisms soil amendments cultivated soils bacteria fungi |
| title | Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments |
| title_full | Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments |
| title_fullStr | Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments |
| title_full_unstemmed | Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments |
| title_short | Isolation of Diverse Phosphate- and Zinc-Solubilizing Microorganisms from Different Environments |
| title_sort | isolation of diverse phosphate and zinc solubilizing microorganisms from different environments |
| topic | P-solubilizing microorganisms Zn-solubilizing microorganisms soil amendments cultivated soils bacteria fungi |
| url | https://www.mdpi.com/2673-8007/4/3/71 |
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