Quantitative, Dynamic Detection of Neuronal Na+ Transients Using Multi-photon Excitation and Fluorescence Lifetime Imaging (FLIM) in Acute Mouse Brain Slices

Quantitative, Dynamic Detection of Neuronal Na+ Transients Using Multi-photon Excitation and Fluorescence Lifetime Imaging (FLIM) in Acute Mouse Brain Slices

Fluorescence lifetime imaging microscopy (FLIM) is a highly valuable technique in the fluorescence microscopy toolbox because it is essentially independent of indicator concentrations. Conventional fluorescence microscopy analyzes changes in emission intensity. In contrast, FLIM assesses the fluores...

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Bibliographic Details
Main Authors: Sara Eitelmann, Karl Kafitz, Christine Rose, Jan Meyer
Format: Article
Language:English
Published: Bio-protocol LLC 2025-02-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5175&type=0
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