Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine

Abstract The soil seed bank (SSB) is a potential resource for the aboveground vegetation community (AVC) and plays a crucial role in ecological restoration. Studying the succession of the SSB and AVC at different restoration stages provides valuable insights into their temporal dynamics, facilitates...

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Main Authors: Yiming Liu, Le Qi, Weibo Ren, Zhe Chen, Feng Yuan, Yaling Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-12056-w
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author Yiming Liu
Le Qi
Weibo Ren
Zhe Chen
Feng Yuan
Yaling Liu
author_facet Yiming Liu
Le Qi
Weibo Ren
Zhe Chen
Feng Yuan
Yaling Liu
author_sort Yiming Liu
collection DOAJ
description Abstract The soil seed bank (SSB) is a potential resource for the aboveground vegetation community (AVC) and plays a crucial role in ecological restoration. Studying the succession of the SSB and AVC at different restoration stages provides valuable insights into their temporal dynamics, facilitates comprehensive investigations of the different restoration stages, and enables appropriate recommendations for the subsequent restoration to be provided. Therefore, the SSB and AVC of restored grassland ecosystems were investigated in open-pit coal mine dumping sites in a typical grassland area of Inner Mongolia. This study was therefore conducted in a typical grassland with the same restoration practices (mixed seeding of Gramineae and Leguminosae), and comparisons were made at different periods post-restoration to determine the pattern of changes in the SSB and AVC over time post-restoration. Through non-metric multidimensional scaling (NMDS), a correlation analysis, and structural equation model, the changes in the SSB and AVC in the years following restoration were determined. It was found that the SSB density was 475.00 − 705.00 (plant m− 2), and the AVC density was 94 − 192.8 (plant m− 2). Notably, a significant shift occurred 17 years post-restoration, indicating a pivotal stage was reached in the overall trajectory of the SSB and AVC. The vegetation in the restored area was found to be transitioning toward natural grassland, with an increase in the proportion of perennial species and a gradual decrease in annual and biannual species. The density of Gramineae and Leguminosae plants was similar to that of natural grasslands 17 years post-restoration. The presence of the invasive species Salsola collina gradually decreased over time. The relationship between soil physicochemical properties and SSB was relatively weak, while the relationship with AVC was relatively strong. With time post-restoration, the resemblance between the SSB and the plant community slowly diminished at the mining dump. The study provides for the prolonged rehabilitation of open-pit coal mine dumping sites.
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spelling doaj-art-907d6a3077c54ce49e77aebca88f7d5c2025-08-20T03:42:25ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-12056-wChanges with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mineYiming Liu0Le Qi1Weibo Ren2Zhe Chen3Feng Yuan4Yaling Liu5Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityInner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityInner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityInner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityKey Laboratory of Forage Breeding and Seed Production of Inner Mongolia, Inner Mongolia M-Grass Ecology and Environment (Group) Co., LtdKey Laboratory of Forage Breeding and Seed Production of Inner Mongolia, Inner Mongolia M-Grass Ecology and Environment (Group) Co., LtdAbstract The soil seed bank (SSB) is a potential resource for the aboveground vegetation community (AVC) and plays a crucial role in ecological restoration. Studying the succession of the SSB and AVC at different restoration stages provides valuable insights into their temporal dynamics, facilitates comprehensive investigations of the different restoration stages, and enables appropriate recommendations for the subsequent restoration to be provided. Therefore, the SSB and AVC of restored grassland ecosystems were investigated in open-pit coal mine dumping sites in a typical grassland area of Inner Mongolia. This study was therefore conducted in a typical grassland with the same restoration practices (mixed seeding of Gramineae and Leguminosae), and comparisons were made at different periods post-restoration to determine the pattern of changes in the SSB and AVC over time post-restoration. Through non-metric multidimensional scaling (NMDS), a correlation analysis, and structural equation model, the changes in the SSB and AVC in the years following restoration were determined. It was found that the SSB density was 475.00 − 705.00 (plant m− 2), and the AVC density was 94 − 192.8 (plant m− 2). Notably, a significant shift occurred 17 years post-restoration, indicating a pivotal stage was reached in the overall trajectory of the SSB and AVC. The vegetation in the restored area was found to be transitioning toward natural grassland, with an increase in the proportion of perennial species and a gradual decrease in annual and biannual species. The density of Gramineae and Leguminosae plants was similar to that of natural grasslands 17 years post-restoration. The presence of the invasive species Salsola collina gradually decreased over time. The relationship between soil physicochemical properties and SSB was relatively weak, while the relationship with AVC was relatively strong. With time post-restoration, the resemblance between the SSB and the plant community slowly diminished at the mining dump. The study provides for the prolonged rehabilitation of open-pit coal mine dumping sites.https://doi.org/10.1038/s41598-025-12056-wVegetation communitySoil seed bankOpen-pit coal mine dumpBiodiversitySimilarity
spellingShingle Yiming Liu
Le Qi
Weibo Ren
Zhe Chen
Feng Yuan
Yaling Liu
Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
Scientific Reports
Vegetation community
Soil seed bank
Open-pit coal mine dump
Biodiversity
Similarity
title Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
title_full Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
title_fullStr Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
title_full_unstemmed Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
title_short Changes with time post-restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open-pit coal mine
title_sort changes with time post restoration in the relationships between soil seed bank and aboveground vegetation in a reclaimed open pit coal mine
topic Vegetation community
Soil seed bank
Open-pit coal mine dump
Biodiversity
Similarity
url https://doi.org/10.1038/s41598-025-12056-w
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