Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i>
To investigate the structure–antioxidant activity relationship, <i>Pleurotus ferulae</i> polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 10<sup>3</sup> kDa, MU-...
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MDPI AG
2025-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/1/91 |
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| author | Hongjin Zhou Zhongxiong Fan Yuan Li Xuelian Liu Bo Wang Jianguo Xing Jiang He Ruifang Zheng Jinyao Li |
| author_facet | Hongjin Zhou Zhongxiong Fan Yuan Li Xuelian Liu Bo Wang Jianguo Xing Jiang He Ruifang Zheng Jinyao Li |
| author_sort | Hongjin Zhou |
| collection | DOAJ |
| description | To investigate the structure–antioxidant activity relationship, <i>Pleurotus ferulae</i> polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 10<sup>3</sup> kDa, MU-PFPS exhibited a lower molecular weight of 89.26 kDa. In addition, unlike U-PFPS, which is primarily composed of glucose (Glu:Man:Gal = 91.1:3.5:5.4), MU-PFPS has a more balanced composition of Glu:Man:Gal in the ratio of 39.4:27.8:32.8 and contains more branched chains. Furthermore, antioxidant analysis revealed that high concentration (at concentrations above 600 μg/mL) MU-PFPS demonstrated stronger protective effects against oxidative damage in RAW264.7 cells than U-PFPS did. Collectively, these data suggest that lower molecular weight and higher branching degree of polysaccharides at appropriate concentrations may correlate with enhanced antioxidant enzyme activities. Our work provides a method for isolating polysaccharides with higher antioxidant activity and offers insights into the structure–activity relationship of polysaccharides, laying the foundation for future applications in polysaccharide modification and structural characterization. |
| format | Article |
| id | doaj-art-90788e180a6649f09a22b8387b4b1b09 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-90788e180a6649f09a22b8387b4b1b092025-01-24T13:19:27ZengMDPI AGAntioxidants2076-39212025-01-011419110.3390/antiox14010091Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i>Hongjin Zhou0Zhongxiong Fan1Yuan Li2Xuelian Liu3Bo Wang4Jianguo Xing5Jiang He6Ruifang Zheng7Jinyao Li8Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Uygur Medical Research, Xinjiang Institute of Materia Medica, Urumqi 830004, ChinaXinjiang Key Laboratory of Uygur Medical Research, Xinjiang Institute of Materia Medica, Urumqi 830004, ChinaXinjiang Key Laboratory of Uygur Medical Research, Xinjiang Institute of Materia Medica, Urumqi 830004, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaTo investigate the structure–antioxidant activity relationship, <i>Pleurotus ferulae</i> polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 10<sup>3</sup> kDa, MU-PFPS exhibited a lower molecular weight of 89.26 kDa. In addition, unlike U-PFPS, which is primarily composed of glucose (Glu:Man:Gal = 91.1:3.5:5.4), MU-PFPS has a more balanced composition of Glu:Man:Gal in the ratio of 39.4:27.8:32.8 and contains more branched chains. Furthermore, antioxidant analysis revealed that high concentration (at concentrations above 600 μg/mL) MU-PFPS demonstrated stronger protective effects against oxidative damage in RAW264.7 cells than U-PFPS did. Collectively, these data suggest that lower molecular weight and higher branching degree of polysaccharides at appropriate concentrations may correlate with enhanced antioxidant enzyme activities. Our work provides a method for isolating polysaccharides with higher antioxidant activity and offers insights into the structure–activity relationship of polysaccharides, laying the foundation for future applications in polysaccharide modification and structural characterization.https://www.mdpi.com/2076-3921/14/1/91structure–activity relationship<i>Pleurotus ferulae</i>polysaccharidesantioxidant activity |
| spellingShingle | Hongjin Zhou Zhongxiong Fan Yuan Li Xuelian Liu Bo Wang Jianguo Xing Jiang He Ruifang Zheng Jinyao Li Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> Antioxidants structure–activity relationship <i>Pleurotus ferulae</i> polysaccharides antioxidant activity |
| title | Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> |
| title_full | Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> |
| title_fullStr | Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> |
| title_full_unstemmed | Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> |
| title_short | Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from <i>Pleurotus ferulae</i> |
| title_sort | structure antioxidant activity relationship of polysaccharides isolated by microwave ultrasonic assisted extraction from i pleurotus ferulae i |
| topic | structure–activity relationship <i>Pleurotus ferulae</i> polysaccharides antioxidant activity |
| url | https://www.mdpi.com/2076-3921/14/1/91 |
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