Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression
Currently, the efficiency of somatic cell nuclear transfer (SCNT) technology is relatively low, primarily owing to reprogramming abnormalities in donor cells or reconstructed embryos. Using histone deacetylase inhibitor (HDACi) to artificially alter the epigenetic modifications of donor cells and im...
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MDPI AG
2025-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/15/7/1022 |
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| author | Xiaoshu Zhe Hairui Ma Wenqi Zhang Rui Ding Fei Hao Yuan Gao Gumara Uri Gellegen Jiri Garangtu Jiri Dongjun Liu |
| author_facet | Xiaoshu Zhe Hairui Ma Wenqi Zhang Rui Ding Fei Hao Yuan Gao Gumara Uri Gellegen Jiri Garangtu Jiri Dongjun Liu |
| author_sort | Xiaoshu Zhe |
| collection | DOAJ |
| description | Currently, the efficiency of somatic cell nuclear transfer (SCNT) technology is relatively low, primarily owing to reprogramming abnormalities in donor cells or reconstructed embryos. Using histone deacetylase inhibitor (HDACi) to artificially alter the epigenetic modifications of donor cells and improve the reprogramming ability of reconstructed embryos is effective in improving nuclear transfer efficiency. In this study, we used Albas cashmere goat cells as donor cells, treated them with Scriptaid, and constructed embryos using SCNT. The results suggest that donor cell treatment with Scriptaid significantly increased the cellular histone acetylation modification level, perturbed the expression of the pluripotency molecule NANOG, altered the reprogramming ability of embryos, and increased the developmental rate of SCNT-reconstructed embryos. Scriptaid inhibited donor cell proliferation, induced apoptosis, and blocked the G0/G1 phase of the cell cycle. These results provide a new research direction for improving SCNT efficiency and a new perspective in the fields of regenerative medicine, agriculture, and animal husbandry. |
| format | Article |
| id | doaj-art-9074e5148b2840bc9f0ae853923fcb21 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-9074e5148b2840bc9f0ae853923fcb212025-08-20T03:06:27ZengMDPI AGAnimals2076-26152025-04-01157102210.3390/ani15071022Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG ExpressionXiaoshu Zhe0Hairui Ma1Wenqi Zhang2Rui Ding3Fei Hao4Yuan Gao5Gumara Uri6Gellegen Jiri7Garangtu Jiri8Dongjun Liu9State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaEtoqqi Agricultural and Animal Husbandry Technology Extension Center, Inner Mongolia, Ordos 016100, ChinaEtoqqi Agricultural and Animal Husbandry Technology Extension Center, Inner Mongolia, Ordos 016100, ChinaEtoqqi Agricultural and Animal Husbandry Technology Extension Center, Inner Mongolia, Ordos 016100, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaCurrently, the efficiency of somatic cell nuclear transfer (SCNT) technology is relatively low, primarily owing to reprogramming abnormalities in donor cells or reconstructed embryos. Using histone deacetylase inhibitor (HDACi) to artificially alter the epigenetic modifications of donor cells and improve the reprogramming ability of reconstructed embryos is effective in improving nuclear transfer efficiency. In this study, we used Albas cashmere goat cells as donor cells, treated them with Scriptaid, and constructed embryos using SCNT. The results suggest that donor cell treatment with Scriptaid significantly increased the cellular histone acetylation modification level, perturbed the expression of the pluripotency molecule NANOG, altered the reprogramming ability of embryos, and increased the developmental rate of SCNT-reconstructed embryos. Scriptaid inhibited donor cell proliferation, induced apoptosis, and blocked the G0/G1 phase of the cell cycle. These results provide a new research direction for improving SCNT efficiency and a new perspective in the fields of regenerative medicine, agriculture, and animal husbandry.https://www.mdpi.com/2076-2615/15/7/1022SCNTScriptaidNANOGhistone acetylationcashmere goat |
| spellingShingle | Xiaoshu Zhe Hairui Ma Wenqi Zhang Rui Ding Fei Hao Yuan Gao Gumara Uri Gellegen Jiri Garangtu Jiri Dongjun Liu Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression Animals SCNT Scriptaid NANOG histone acetylation cashmere goat |
| title | Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression |
| title_full | Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression |
| title_fullStr | Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression |
| title_full_unstemmed | Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression |
| title_short | Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression |
| title_sort | scriptaid improves cashmere goat embryo reprogramming by affecting donor cell pluripotency molecule nanog expression |
| topic | SCNT Scriptaid NANOG histone acetylation cashmere goat |
| url | https://www.mdpi.com/2076-2615/15/7/1022 |
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