Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro
Chromogranin B and other members of the granin protein family form condensates that recruit clients like proinsulin. The condensation in the lumen of trans-Golgi network (TGN) is critical for the biogenesis of secretory granules. Here, we describe a protocol to purify the tagged version of chromogra...
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| Format: | Article |
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Bio-protocol LLC
2024-10-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5095&type=0 |
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| author | Anup Parchure Julia von Blume |
| author_facet | Anup Parchure Julia von Blume |
| author_sort | Anup Parchure |
| collection | DOAJ |
| description | Chromogranin B and other members of the granin protein family form condensates that recruit clients like proinsulin. The condensation in the lumen of trans-Golgi network (TGN) is critical for the biogenesis of secretory granules. Here, we describe a protocol to purify the tagged version of chromogranin B close to its native form at the TGN, which can then be utilized for microscopy-based assays to monitor condensate formation in vitro and client partitioning depending on the material properties of chromogranin B assemblies. |
| format | Article |
| id | doaj-art-906a0d88bf7f403c888f45c45e5fdaf8 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-906a0d88bf7f403c888f45c45e5fdaf82025-08-20T01:57:24ZengBio-protocol LLCBio-Protocol2331-83252024-10-01142010.21769/BioProtoc.5095Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In VitroAnup Parchure0Julia von Blume1Section of Endocrinology, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USADepartment of Cell Biology, Yale School of Medicine, New Haven, CT, USADepartment of Cell Biology, Yale School of Medicine, New Haven, CT, USAChromogranin B and other members of the granin protein family form condensates that recruit clients like proinsulin. The condensation in the lumen of trans-Golgi network (TGN) is critical for the biogenesis of secretory granules. Here, we describe a protocol to purify the tagged version of chromogranin B close to its native form at the TGN, which can then be utilized for microscopy-based assays to monitor condensate formation in vitro and client partitioning depending on the material properties of chromogranin B assemblies.https://bio-protocol.org/en/bpdetail?id=5095&type=0 |
| spellingShingle | Anup Parchure Julia von Blume Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro Bio-Protocol |
| title | Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro |
| title_full | Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro |
| title_fullStr | Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro |
| title_full_unstemmed | Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro |
| title_short | Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro |
| title_sort | chromogranin b purification for condensate formation and client partitioning assays in vitro |
| url | https://bio-protocol.org/en/bpdetail?id=5095&type=0 |
| work_keys_str_mv | AT anupparchure chromograninbpurificationforcondensateformationandclientpartitioningassaysinvitro AT juliavonblume chromograninbpurificationforcondensateformationandclientpartitioningassaysinvitro |