Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis
Abstract Inflammatory bowel disease (IBD) is a chronic, relapsing, and remitting disease characterized by chronic inflammation in the gastrointestinal tract. The exact etiology and pathogenesis of IBD remain elusive. Although ELF-1 has been known to be highly expressed in epithelial cells for past t...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07742-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850028013103611904 |
|---|---|
| author | Gege He Pingping Liu Xiaoyan Xuan Min Zhang Hongxia Zhang Ka Yang Yusheng Luan Qian Yang Jingyuan Yang Qianru Li Huaixin Zheng Peng Wang |
| author_facet | Gege He Pingping Liu Xiaoyan Xuan Min Zhang Hongxia Zhang Ka Yang Yusheng Luan Qian Yang Jingyuan Yang Qianru Li Huaixin Zheng Peng Wang |
| author_sort | Gege He |
| collection | DOAJ |
| description | Abstract Inflammatory bowel disease (IBD) is a chronic, relapsing, and remitting disease characterized by chronic inflammation in the gastrointestinal tract. The exact etiology and pathogenesis of IBD remain elusive. Although ELF-1 has been known to be highly expressed in epithelial cells for past twenty years, little is known about its function in epithelial cells and epithelial-related IBD. Here, we demonstrated that ELF-1 deficiency in mouse lead to exacerbated DSS-induced colitis, marked by inflammation dominated by neutrophil infiltration and activation of IL-17 signaling pathways in various immune cells, including Th17, ILC3, γδT and NKT cells. Bone marrow transfer experiments confirmed ELF-1 deficiency in non-hematopoietic cells intrinsically worsened DSS-induced colitis. On one hand, ELF-1 deficiency enhanced the production of pro-inflammatory chemokines in colonic epithelial cells, leading to extensive infiltration of neutrophils and other immune cells into the colonic mucosal tissue. On the other hand, ELF-1 directly regulated the expression of the Rack1 gene in colonic epithelial tissue, which has been proved to play critical roles in maintaining intestinal homeostasis. Altogether, ELF-1 plays a protective role in colitis by maintaining intestinal epithelium homeostasis. |
| format | Article |
| id | doaj-art-90649d52bd7141b7b74606d8b63f45f7 |
| institution | DOAJ |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-90649d52bd7141b7b74606d8b63f45f72025-08-20T02:59:57ZengNature PortfolioCommunications Biology2399-36422025-03-018111210.1038/s42003-025-07742-4Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasisGege He0Pingping Liu1Xiaoyan Xuan2Min Zhang3Hongxia Zhang4Ka Yang5Yusheng Luan6Qian Yang7Jingyuan Yang8Qianru Li9Huaixin Zheng10Peng Wang11Department of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Zhengzhou UniversityAbstract Inflammatory bowel disease (IBD) is a chronic, relapsing, and remitting disease characterized by chronic inflammation in the gastrointestinal tract. The exact etiology and pathogenesis of IBD remain elusive. Although ELF-1 has been known to be highly expressed in epithelial cells for past twenty years, little is known about its function in epithelial cells and epithelial-related IBD. Here, we demonstrated that ELF-1 deficiency in mouse lead to exacerbated DSS-induced colitis, marked by inflammation dominated by neutrophil infiltration and activation of IL-17 signaling pathways in various immune cells, including Th17, ILC3, γδT and NKT cells. Bone marrow transfer experiments confirmed ELF-1 deficiency in non-hematopoietic cells intrinsically worsened DSS-induced colitis. On one hand, ELF-1 deficiency enhanced the production of pro-inflammatory chemokines in colonic epithelial cells, leading to extensive infiltration of neutrophils and other immune cells into the colonic mucosal tissue. On the other hand, ELF-1 directly regulated the expression of the Rack1 gene in colonic epithelial tissue, which has been proved to play critical roles in maintaining intestinal homeostasis. Altogether, ELF-1 plays a protective role in colitis by maintaining intestinal epithelium homeostasis.https://doi.org/10.1038/s42003-025-07742-4 |
| spellingShingle | Gege He Pingping Liu Xiaoyan Xuan Min Zhang Hongxia Zhang Ka Yang Yusheng Luan Qian Yang Jingyuan Yang Qianru Li Huaixin Zheng Peng Wang Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis Communications Biology |
| title | Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis |
| title_full | Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis |
| title_fullStr | Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis |
| title_full_unstemmed | Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis |
| title_short | Transcription factor ELF-1 protects against colitis by maintaining intestinal epithelium homeostasis |
| title_sort | transcription factor elf 1 protects against colitis by maintaining intestinal epithelium homeostasis |
| url | https://doi.org/10.1038/s42003-025-07742-4 |
| work_keys_str_mv | AT gegehe transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT pingpingliu transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT xiaoyanxuan transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT minzhang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT hongxiazhang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT kayang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT yushengluan transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT qianyang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT jingyuanyang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT qianruli transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT huaixinzheng transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis AT pengwang transcriptionfactorelf1protectsagainstcolitisbymaintainingintestinalepitheliumhomeostasis |