Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4
The increasing prevalence of LED technology heightened blue light (BL) exposure, raising concerns about its long-term effects on ocular health. This study investigated the transcriptomic response of conjunctiva to BL exposure, highlighting potential biomarkers for conjunctival injury. We exposed hum...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016609 |
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| _version_ | 1823856918418948096 |
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| author | Qianjie Yang Kuangqi Chen Siyi Chen Yinhao Wang Yutong Xia Jinbo Chen Ye Shen |
| author_facet | Qianjie Yang Kuangqi Chen Siyi Chen Yinhao Wang Yutong Xia Jinbo Chen Ye Shen |
| author_sort | Qianjie Yang |
| collection | DOAJ |
| description | The increasing prevalence of LED technology heightened blue light (BL) exposure, raising concerns about its long-term effects on ocular health. This study investigated the transcriptomic response of conjunctiva to BL exposure, highlighting potential biomarkers for conjunctival injury. We exposed human conjunctival epithelial cells and C57BL/6 mice to BL to establish in vitro and in vivo models and identified the responsive genes in mice’s conjunctiva to BL exposure by RNA sequencing transcriptome analysis. Western blotting, wound healing assays, transwell assay, and phalloidin staining assessed phenotypes of epithelial-mesenchymal transition (EMT). BL disrupted cell conjunction and regulated EMT-related proteins. RNA sequencing analysis revealed upregulation of ITGB4 and enrichment of cell migration and adhesion pathways. Reactive oxygen species-mediated damage caused by BL upregulated ITGB4 expression, promoting cell migration and EMT through the extracellular signal-regulated kinase /Snail pathway. |
| format | Article |
| id | doaj-art-90512ecc71a64cefa5b10f7f823cb16d |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-90512ecc71a64cefa5b10f7f823cb16d2025-02-12T05:29:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117584Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4Qianjie Yang0Kuangqi Chen1Siyi Chen2Yinhao Wang3Yutong Xia4Jinbo Chen5Ye Shen6Ningbo Eye Institute, Ningbo Eye Hospital, Wenzhou Medical University, Ningbo, Zhejiang Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, ChinaAlfred Health, Melbourne, AustraliaDepartment of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, ChinaNingbo Eye Institute, Ningbo Eye Hospital, Wenzhou Medical University, Ningbo, Zhejiang Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, China; Corresponding author.The increasing prevalence of LED technology heightened blue light (BL) exposure, raising concerns about its long-term effects on ocular health. This study investigated the transcriptomic response of conjunctiva to BL exposure, highlighting potential biomarkers for conjunctival injury. We exposed human conjunctival epithelial cells and C57BL/6 mice to BL to establish in vitro and in vivo models and identified the responsive genes in mice’s conjunctiva to BL exposure by RNA sequencing transcriptome analysis. Western blotting, wound healing assays, transwell assay, and phalloidin staining assessed phenotypes of epithelial-mesenchymal transition (EMT). BL disrupted cell conjunction and regulated EMT-related proteins. RNA sequencing analysis revealed upregulation of ITGB4 and enrichment of cell migration and adhesion pathways. Reactive oxygen species-mediated damage caused by BL upregulated ITGB4 expression, promoting cell migration and EMT through the extracellular signal-regulated kinase /Snail pathway.http://www.sciencedirect.com/science/article/pii/S0147651324016609EMTblue lightconjunctival epitheliummigrationITGB4 |
| spellingShingle | Qianjie Yang Kuangqi Chen Siyi Chen Yinhao Wang Yutong Xia Jinbo Chen Ye Shen Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 Ecotoxicology and Environmental Safety EMT blue light conjunctival epithelium migration ITGB4 |
| title | Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 |
| title_full | Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 |
| title_fullStr | Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 |
| title_full_unstemmed | Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 |
| title_short | Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 |
| title_sort | blue light promotes conjunctival epithelial mesenchymal transition and collagen deposition through itgb4 |
| topic | EMT blue light conjunctival epithelium migration ITGB4 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016609 |
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