Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome

Myelodysplastic syndrome (MDS) is a clonal hematopoietic stem cell disease. Supportive treatments, such as erythropoiesis-stimulating agents (ESAs), are commonly used in patients with low-risk MDS. This study aimed to retrospectively assess the impact of bone marrow Mothers against decapentaplegic...

Full description

Saved in:
Bibliographic Details
Main Authors: Bedrettin Orhan, Hülya Öztürk Nazlıoğlu, Oğuzhan Dik, Büşra Gürbüz, Vildan Özkocaman, Tuba Ersal, İbrahim Ethem Pınar Pınar, Cumali Yalçın Yalçın, Sinem Çubukçu, Tuba Güllü Koca, Fazıl Çağrı Hunutlu, Şeyma Yavuz, Rıdvan Ali, Fahir Özkalemkaş
Format: Article
Language:English
Published: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2025-03-01
Series:Biomolecules & Biomedicine
Subjects:
Online Access:https://www.bjbms.org/ojs/index.php/bjbms/article/view/11564
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850030526026481664
author Bedrettin Orhan
Hülya Öztürk Nazlıoğlu
Oğuzhan Dik
Büşra Gürbüz
Vildan Özkocaman
Tuba Ersal
İbrahim Ethem Pınar Pınar
Cumali Yalçın Yalçın
Sinem Çubukçu
Tuba Güllü Koca
Fazıl Çağrı Hunutlu
Şeyma Yavuz
Rıdvan Ali
Fahir Özkalemkaş
author_facet Bedrettin Orhan
Hülya Öztürk Nazlıoğlu
Oğuzhan Dik
Büşra Gürbüz
Vildan Özkocaman
Tuba Ersal
İbrahim Ethem Pınar Pınar
Cumali Yalçın Yalçın
Sinem Çubukçu
Tuba Güllü Koca
Fazıl Çağrı Hunutlu
Şeyma Yavuz
Rıdvan Ali
Fahir Özkalemkaş
author_sort Bedrettin Orhan
collection DOAJ
description Myelodysplastic syndrome (MDS) is a clonal hematopoietic stem cell disease. Supportive treatments, such as erythropoiesis-stimulating agents (ESAs), are commonly used in patients with low-risk MDS. This study aimed to retrospectively assess the impact of bone marrow Mothers against decapentaplegic homolog 7 (SMAD-7) and transforming growth factor beta 1 (TGF-β1) protein expression on prognosis and response to ESA treatment in patients with low-risk MDS. We retrospectively analyzed patients diagnosed with low-risk MDS at the adult hematology department of Bursa Uludağ University Hospital. A total of 56 patients classified as low or very low risk were included in the study. Immunohistochemical analysis of bone marrow specimens at diagnosis showed that only five patients (9.8%) exhibited low SMAD-7 staining, while 51 patients (90.2%) showed no staining. Regarding TGF-β1 staining, 18 patients (32.1%) demonstrated moderate to high staining, whereas 38 patients (67.9%) exhibited low (36/38) or no staining (2/38). A statistically significant correlation was found between TGF-β1 staining levels and ESA treatment administration (P = 0.011). Additionally, a significant relationship was observed between lower erythropoietin (EPO) levels and moderate to high TGF-β1 staining (P = 0.04). However, when TGF-β1 staining status was compared with first- and third-month treatment responses in patients receiving ESA therapy, no significant difference was detected between groups. These findings suggest that while TGF-β1 alone may not be sufficient to predict ESA treatment response, additional parameters related to the TGF-β/SMAD pathway should be considered. Strong TGF-β1 staining, alongside EPO levels, may influence the decision to initiate ESA therapy.
format Article
id doaj-art-904bf3bf92fb4e4c84a58ba394b8638c
institution DOAJ
issn 2831-0896
2831-090X
language English
publishDate 2025-03-01
publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
record_format Article
series Biomolecules & Biomedicine
spelling doaj-art-904bf3bf92fb4e4c84a58ba394b8638c2025-08-20T02:59:12ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-03-0110.17305/bb.2025.11564Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndromeBedrettin Orhan0https://orcid.org/0000-0003-3970-2344Hülya Öztürk Nazlıoğlu1Oğuzhan Dik2https://orcid.org/0009-0006-3203-5568Büşra Gürbüz3Vildan Özkocaman4Tuba Ersal5İbrahim Ethem Pınar Pınar6https://orcid.org/0000-0001-9907-1498Cumali Yalçın Yalçın7https://orcid.org/0000-0002-5129-2977Sinem Çubukçu8Tuba Güllü Koca9Fazıl Çağrı Hunutlu10https://orcid.org/0000-0002-4991-9830Şeyma Yavuz11Rıdvan Ali12Fahir Özkalemkaş13Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDepartment of Pathology, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDepartment of Pathology, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDepartment of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, TürkiyeDivision of Hematology, Department of Internal Medicine, Faculty of Medicine, Bursa Uludag University, Bursa, Türkiye Myelodysplastic syndrome (MDS) is a clonal hematopoietic stem cell disease. Supportive treatments, such as erythropoiesis-stimulating agents (ESAs), are commonly used in patients with low-risk MDS. This study aimed to retrospectively assess the impact of bone marrow Mothers against decapentaplegic homolog 7 (SMAD-7) and transforming growth factor beta 1 (TGF-β1) protein expression on prognosis and response to ESA treatment in patients with low-risk MDS. We retrospectively analyzed patients diagnosed with low-risk MDS at the adult hematology department of Bursa Uludağ University Hospital. A total of 56 patients classified as low or very low risk were included in the study. Immunohistochemical analysis of bone marrow specimens at diagnosis showed that only five patients (9.8%) exhibited low SMAD-7 staining, while 51 patients (90.2%) showed no staining. Regarding TGF-β1 staining, 18 patients (32.1%) demonstrated moderate to high staining, whereas 38 patients (67.9%) exhibited low (36/38) or no staining (2/38). A statistically significant correlation was found between TGF-β1 staining levels and ESA treatment administration (P = 0.011). Additionally, a significant relationship was observed between lower erythropoietin (EPO) levels and moderate to high TGF-β1 staining (P = 0.04). However, when TGF-β1 staining status was compared with first- and third-month treatment responses in patients receiving ESA therapy, no significant difference was detected between groups. These findings suggest that while TGF-β1 alone may not be sufficient to predict ESA treatment response, additional parameters related to the TGF-β/SMAD pathway should be considered. Strong TGF-β1 staining, alongside EPO levels, may influence the decision to initiate ESA therapy. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11564Myelodysplastic syndromeMDSMothers against decapentaplegic homolog 7SMAD-7Transforming Growth Factor Beta 1TGF-β1
spellingShingle Bedrettin Orhan
Hülya Öztürk Nazlıoğlu
Oğuzhan Dik
Büşra Gürbüz
Vildan Özkocaman
Tuba Ersal
İbrahim Ethem Pınar Pınar
Cumali Yalçın Yalçın
Sinem Çubukçu
Tuba Güllü Koca
Fazıl Çağrı Hunutlu
Şeyma Yavuz
Rıdvan Ali
Fahir Özkalemkaş
Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
Biomolecules & Biomedicine
Myelodysplastic syndrome
MDS
Mothers against decapentaplegic homolog 7
SMAD-7
Transforming Growth Factor Beta 1
TGF-β1
title Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
title_full Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
title_fullStr Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
title_full_unstemmed Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
title_short Predictive value of TGF-β1 and SMAD-7 expression at diagnosis for treatment response in low-risk myelodysplastic syndrome
title_sort predictive value of tgf β1 and smad 7 expression at diagnosis for treatment response in low risk myelodysplastic syndrome
topic Myelodysplastic syndrome
MDS
Mothers against decapentaplegic homolog 7
SMAD-7
Transforming Growth Factor Beta 1
TGF-β1
url https://www.bjbms.org/ojs/index.php/bjbms/article/view/11564
work_keys_str_mv AT bedrettinorhan predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT hulyaozturknazlıoglu predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT oguzhandik predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT busragurbuz predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT vildanozkocaman predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT tubaersal predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT ibrahimethempınarpınar predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT cumaliyalcınyalcın predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT sinemcubukcu predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT tubagullukoca predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT fazılcagrıhunutlu predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT seymayavuz predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT rıdvanali predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome
AT fahirozkalemkas predictivevalueoftgfb1andsmad7expressionatdiagnosisfortreatmentresponseinlowriskmyelodysplasticsyndrome