Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation
One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/283085 |
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| author | Ji Weng Yuning Liu Ying Xu Ruiqi Hu Haolin Zhang Xia Sheng Gen Watanabe Kazuyoshi Taya Qiang Weng Meiyu Xu |
| author_facet | Ji Weng Yuning Liu Ying Xu Ruiqi Hu Haolin Zhang Xia Sheng Gen Watanabe Kazuyoshi Taya Qiang Weng Meiyu Xu |
| author_sort | Ji Weng |
| collection | DOAJ |
| description | One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation. |
| format | Article |
| id | doaj-art-904b604303824f288442b9146256c19f |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-904b604303824f288442b9146256c19f2025-02-03T07:25:10ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/283085283085Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during PrehibernationJi Weng0Yuning Liu1Ying Xu2Ruiqi Hu3Haolin Zhang4Xia Sheng5Gen Watanabe6Kazuyoshi Taya7Qiang Weng8Meiyu Xu9College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaLaboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanLaboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaOne specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.http://dx.doi.org/10.1155/2015/283085 |
| spellingShingle | Ji Weng Yuning Liu Ying Xu Ruiqi Hu Haolin Zhang Xia Sheng Gen Watanabe Kazuyoshi Taya Qiang Weng Meiyu Xu Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation International Journal of Endocrinology |
| title | Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation |
| title_full | Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation |
| title_fullStr | Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation |
| title_full_unstemmed | Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation |
| title_short | Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation |
| title_sort | expression of p450arom and estrogen receptor alpha in the oviduct of chinese brown frog rana dybowskii during prehibernation |
| url | http://dx.doi.org/10.1155/2015/283085 |
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