Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora
Soft rot is an important potato disease caused by the bacterial pathogen Erwinia carotovora. Although chemical bactericides are commonly used for control, biological control using antagonistic bacteria offers a promising and environmentally friendly alternative. Bacteria capable of surviving in ext...
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| Format: | Article |
| Language: | English |
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Universitas Andalas
2025-07-01
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| Series: | Jurnal Proteksi Tanaman |
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| Online Access: | https://jpt.faperta.unand.ac.id/index.php/jpt/article/view/268 |
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| author | Ahmad Muhidin Femita Hapsari Luqman Qurata Aini Muhammad Akhid Syib’li Restu Rizkyta Kusuma |
| author_facet | Ahmad Muhidin Femita Hapsari Luqman Qurata Aini Muhammad Akhid Syib’li Restu Rizkyta Kusuma |
| author_sort | Ahmad Muhidin |
| collection | DOAJ |
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Soft rot is an important potato disease caused by the bacterial pathogen Erwinia carotovora. Although chemical bactericides are commonly used for control, biological control using antagonistic bacteria offers a promising and environmentally friendly alternative. Bacteria capable of surviving in extreme environments have been reported to show high efficiency and the ability to withstand various environmental conditions. This study aimed to isolate, evaluate the potential and activity, and identify the molecular basis of novel bacteria from Sidoarjo hot mud against E. carotovora, the causal agent of potato soft rot. The methods used included sample collection, bacterial isolation and screening, inhibition assays against E. carotovora, soft rot suppression assays on potato tubers, molecular identification using 16S rRNA gene sequencing, and antibiosis mechanism testing. Based on the research, 16 bacterial isolates were obtained, of which four isolates (BLS3, BLS6, BLS7, and BLS10) demonstrated antagonistic activity, as evidenced by the formation of clear inhibition zones on nutrient agar medium. Suppression assays further confirmed their ability to significantly reduce soft rot symptoms in potato tubers. Among these, BLS10 isolate exhibited the highest inhibition and suppression activity, with an inhibition zone of 2.03 cm and a soft rot mass of 0.52 grams. Molecular identification based on 16S rRNA gene sequencing showed that isolates of BLS3, BLS6, BLS7, and BLS10 were identified respectively as Bacillus velezensis, Bacillus methylotrophicus, Bacillus amyloliquefaciens, and Ochrobactrum intermedium. All isolates demonstrated a bacteriostatic antibiosis mechanism, indicated by turbidity in peptone water, suggesting inhibition through secondary metabolite production.
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| format | Article |
| id | doaj-art-904550dc2a34401f9245f5ee43237bfb |
| institution | Kabale University |
| issn | 2580-0604 2621-3141 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Universitas Andalas |
| record_format | Article |
| series | Jurnal Proteksi Tanaman |
| spelling | doaj-art-904550dc2a34401f9245f5ee43237bfb2025-08-20T03:28:59ZengUniversitas AndalasJurnal Proteksi Tanaman2580-06042621-31412025-07-019110.25077/jpt.9.1.58-71.2025Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovoraAhmad Muhidin0Femita HapsariLuqman Qurata AiniMuhammad Akhid Syib’liRestu Rizkyta KusumaUniversitas Brawijaya Soft rot is an important potato disease caused by the bacterial pathogen Erwinia carotovora. Although chemical bactericides are commonly used for control, biological control using antagonistic bacteria offers a promising and environmentally friendly alternative. Bacteria capable of surviving in extreme environments have been reported to show high efficiency and the ability to withstand various environmental conditions. This study aimed to isolate, evaluate the potential and activity, and identify the molecular basis of novel bacteria from Sidoarjo hot mud against E. carotovora, the causal agent of potato soft rot. The methods used included sample collection, bacterial isolation and screening, inhibition assays against E. carotovora, soft rot suppression assays on potato tubers, molecular identification using 16S rRNA gene sequencing, and antibiosis mechanism testing. Based on the research, 16 bacterial isolates were obtained, of which four isolates (BLS3, BLS6, BLS7, and BLS10) demonstrated antagonistic activity, as evidenced by the formation of clear inhibition zones on nutrient agar medium. Suppression assays further confirmed their ability to significantly reduce soft rot symptoms in potato tubers. Among these, BLS10 isolate exhibited the highest inhibition and suppression activity, with an inhibition zone of 2.03 cm and a soft rot mass of 0.52 grams. Molecular identification based on 16S rRNA gene sequencing showed that isolates of BLS3, BLS6, BLS7, and BLS10 were identified respectively as Bacillus velezensis, Bacillus methylotrophicus, Bacillus amyloliquefaciens, and Ochrobactrum intermedium. All isolates demonstrated a bacteriostatic antibiosis mechanism, indicated by turbidity in peptone water, suggesting inhibition through secondary metabolite production. https://jpt.faperta.unand.ac.id/index.php/jpt/article/view/268Antagonistic bacteriabiological controlBLS10inhibitionOchrobactrum intermedium |
| spellingShingle | Ahmad Muhidin Femita Hapsari Luqman Qurata Aini Muhammad Akhid Syib’li Restu Rizkyta Kusuma Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora Jurnal Proteksi Tanaman Antagonistic bacteria biological control BLS10 inhibition Ochrobactrum intermedium |
| title | Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora |
| title_full | Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora |
| title_fullStr | Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora |
| title_full_unstemmed | Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora |
| title_short | Potential of Novel Bacteria from Sidoarjo Hot Mud for Controlling Potato Soft Rot Caused by Erwinia carotovora |
| title_sort | potential of novel bacteria from sidoarjo hot mud for controlling potato soft rot caused by erwinia carotovora |
| topic | Antagonistic bacteria biological control BLS10 inhibition Ochrobactrum intermedium |
| url | https://jpt.faperta.unand.ac.id/index.php/jpt/article/view/268 |
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