Optogenetic control of mitochondrial aggregation and function
The balance of mitochondrial fission and fusion plays an important role in maintaining the stability of cellular homeostasis. Abnormal mitochondrial fission and fragmentation have been shown to be associated with oxidative stress, which causes a variety of human diseases from neurodegeneration disea...
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| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1500343/full |
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| author | Luhao Zhang Luhao Zhang Luhao Zhang Xuechun Liu Min Zhu Min Zhu Yuanfa Yao Zhichao Liu Xianming Zhang Xin Deng Yi Wang Liting Duan Xiaogang Guo Junfen Fu Yingke Xu Yingke Xu Yingke Xu |
| author_facet | Luhao Zhang Luhao Zhang Luhao Zhang Xuechun Liu Min Zhu Min Zhu Yuanfa Yao Zhichao Liu Xianming Zhang Xin Deng Yi Wang Liting Duan Xiaogang Guo Junfen Fu Yingke Xu Yingke Xu Yingke Xu |
| author_sort | Luhao Zhang |
| collection | DOAJ |
| description | The balance of mitochondrial fission and fusion plays an important role in maintaining the stability of cellular homeostasis. Abnormal mitochondrial fission and fragmentation have been shown to be associated with oxidative stress, which causes a variety of human diseases from neurodegeneration disease to cancer. Therefore, the induction of mitochondrial aggregation and fusion may provide an alternative approach to alleviate these conditions. Here, an optogenetic-based mitochondrial aggregation system (Opto-MitoA) developed, which is based on the CRY2clust/CIBN light-sensitive module. Upon blue light illumination, CRY2clust relocates from the cytosol to mitochondria where it induces mitochondrial aggregation by CRY2clust homo-oligomerization and CRY2clust-CIBN hetero-dimerization. Our functional experiments demonstrate that Opto-MitoA-induced mitochondrial aggregation potently alleviates niclosamide-caused cell dysfunction in ATP production. This study establishes a novel optogenetic-based strategy to regulate mitochondrial dynamics in cells, which may provide a potential therapy for treating mitochondrial-related diseases. |
| format | Article |
| id | doaj-art-90249608858d4c3f8b0355dab6cd7684 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-90249608858d4c3f8b0355dab6cd76842025-01-06T06:59:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15003431500343Optogenetic control of mitochondrial aggregation and functionLuhao Zhang0Luhao Zhang1Luhao Zhang2Xuechun Liu3Min Zhu4Min Zhu5Yuanfa Yao6Zhichao Liu7Xianming Zhang8Xin Deng9Yi Wang10Liting Duan11Xiaogang Guo12Junfen Fu13Yingke Xu14Yingke Xu15Yingke Xu16Department of Endocrinology, Children’s Hospital of Zhejiang University School of Medicine, National Clinical Research Center for Children’s Health, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaInnovation Center for Smart Medical Technologies and Devices, Binjiang Institute of Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaDepartment of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaDepartment of Endocrinology, Children’s Hospital of Zhejiang University School of Medicine, National Clinical Research Center for Children’s Health, Hangzhou, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, ChinaDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Sha Tin, Hong Kong SAR, ChinaDepartment of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Endocrinology, Children’s Hospital of Zhejiang University School of Medicine, National Clinical Research Center for Children’s Health, Hangzhou, ChinaDepartment of Endocrinology, Children’s Hospital of Zhejiang University School of Medicine, National Clinical Research Center for Children’s Health, Hangzhou, ChinaDepartment of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, ChinaInnovation Center for Smart Medical Technologies and Devices, Binjiang Institute of Zhejiang University, Hangzhou, ChinaThe balance of mitochondrial fission and fusion plays an important role in maintaining the stability of cellular homeostasis. Abnormal mitochondrial fission and fragmentation have been shown to be associated with oxidative stress, which causes a variety of human diseases from neurodegeneration disease to cancer. Therefore, the induction of mitochondrial aggregation and fusion may provide an alternative approach to alleviate these conditions. Here, an optogenetic-based mitochondrial aggregation system (Opto-MitoA) developed, which is based on the CRY2clust/CIBN light-sensitive module. Upon blue light illumination, CRY2clust relocates from the cytosol to mitochondria where it induces mitochondrial aggregation by CRY2clust homo-oligomerization and CRY2clust-CIBN hetero-dimerization. Our functional experiments demonstrate that Opto-MitoA-induced mitochondrial aggregation potently alleviates niclosamide-caused cell dysfunction in ATP production. This study establishes a novel optogenetic-based strategy to regulate mitochondrial dynamics in cells, which may provide a potential therapy for treating mitochondrial-related diseases.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1500343/fulloptogeneticsmitochondriaaggregationATPimaging |
| spellingShingle | Luhao Zhang Luhao Zhang Luhao Zhang Xuechun Liu Min Zhu Min Zhu Yuanfa Yao Zhichao Liu Xianming Zhang Xin Deng Yi Wang Liting Duan Xiaogang Guo Junfen Fu Yingke Xu Yingke Xu Yingke Xu Optogenetic control of mitochondrial aggregation and function Frontiers in Bioengineering and Biotechnology optogenetics mitochondria aggregation ATP imaging |
| title | Optogenetic control of mitochondrial aggregation and function |
| title_full | Optogenetic control of mitochondrial aggregation and function |
| title_fullStr | Optogenetic control of mitochondrial aggregation and function |
| title_full_unstemmed | Optogenetic control of mitochondrial aggregation and function |
| title_short | Optogenetic control of mitochondrial aggregation and function |
| title_sort | optogenetic control of mitochondrial aggregation and function |
| topic | optogenetics mitochondria aggregation ATP imaging |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1500343/full |
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