High-throughput characterisation of bull semen motility using differential dynamic microscopy.
We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm2) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 106 cells/ml) at once and yields averaged measurement...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202720&type=printable |
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| author | Alys Jepson Jochen Arlt Jonathan Statham Mark Spilman Katie Burton Tiffany Wood Wilson C K Poon Vincent A Martinez |
| author_facet | Alys Jepson Jochen Arlt Jonathan Statham Mark Spilman Katie Burton Tiffany Wood Wilson C K Poon Vincent A Martinez |
| author_sort | Alys Jepson |
| collection | DOAJ |
| description | We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm2) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 106 cells/ml) at once and yields averaged measurements of the mean ([Formula: see text]) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A0) and frequency (f0), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Formula: see text] for swimming spermatozoa. |
| format | Article |
| id | doaj-art-90117b8c3d2541ca8bbcb12c07daf512 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-90117b8c3d2541ca8bbcb12c07daf5122025-08-20T02:04:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01144e020272010.1371/journal.pone.0202720High-throughput characterisation of bull semen motility using differential dynamic microscopy.Alys JepsonJochen ArltJonathan StathamMark SpilmanKatie BurtonTiffany WoodWilson C K PoonVincent A MartinezWe report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm2) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 106 cells/ml) at once and yields averaged measurements of the mean ([Formula: see text]) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A0) and frequency (f0), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Formula: see text] for swimming spermatozoa.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202720&type=printable |
| spellingShingle | Alys Jepson Jochen Arlt Jonathan Statham Mark Spilman Katie Burton Tiffany Wood Wilson C K Poon Vincent A Martinez High-throughput characterisation of bull semen motility using differential dynamic microscopy. PLoS ONE |
| title | High-throughput characterisation of bull semen motility using differential dynamic microscopy. |
| title_full | High-throughput characterisation of bull semen motility using differential dynamic microscopy. |
| title_fullStr | High-throughput characterisation of bull semen motility using differential dynamic microscopy. |
| title_full_unstemmed | High-throughput characterisation of bull semen motility using differential dynamic microscopy. |
| title_short | High-throughput characterisation of bull semen motility using differential dynamic microscopy. |
| title_sort | high throughput characterisation of bull semen motility using differential dynamic microscopy |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202720&type=printable |
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