Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin
In vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with <i>Cannabis sativa</i> (‘Cherry1’, ‘BaOx’, ‘T1’, ‘Peach’) grown in vessels...
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MDPI AG
2025-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/7/1138 |
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| author | Molly McKay James E. Faust Matthew Taylor Jeffrey Adelberg |
| author_facet | Molly McKay James E. Faust Matthew Taylor Jeffrey Adelberg |
| author_sort | Molly McKay |
| collection | DOAJ |
| description | In vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with <i>Cannabis sativa</i> (‘Cherry1’, ‘BaOx’, ‘T1’, ‘Peach’) grown in vessels of three different physical states—stationary agar (A); stationary Oasis<sup>®</sup> infused with liquid (OILs); and agitated Oasis<sup>®</sup> infused with liquid (OILa). Vessels were pre-selected as control or supplemented; where supplement vessels received 15 mL DKW liquid media each cycle harvest. The number of shoot tips harvested; shoot length; and dry shoot mass from repeated cutting cycles was recorded. In a single harvest; ‘BaOx’ and ‘Cherry 1’ produced one shoot per plant from the original 15 planted on all treatments. ‘Peach’ and ‘T1’ produced less shoots on average; but the most in OIL treatments. All shoots harvested were longer in OIL compared to A; regardless of genotype. Over multiple cycles; ‘Peach’ and ‘T1’ were unable to reliably produce shoots on a repeated schedule and were, therefore, eliminated from the experiment. By cycle 3; maximum number of plants were produced; regardless of supplementation (‘Cherry 1’; 30; ‘BaOx’; 22). Shoot length was above 10 mm (planting standard) for both genotypes until after the third cycle (10 weeks) where number and quality decreased (nodes and internodes easily discerned). By the end of the experiment; the only shoots that remained productive for over 16 weeks and multiple repeated harvest cycles were those in OIL treatments with supplements. |
| format | Article |
| id | doaj-art-900f3a056b2d4d5486913fd4acf702c2 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-900f3a056b2d4d5486913fd4acf702c22025-08-20T03:03:25ZengMDPI AGPlants2223-77472025-04-01147113810.3390/plants14071138Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of CytokininMolly McKay0James E. Faust1Matthew Taylor2Jeffrey Adelberg3Plant and Environmental Science Department, 171 Poole Agricultural Center, Clemson University, Clemson, SC 29630, USAPlant and Environmental Science Department, 171 Poole Agricultural Center, Clemson University, Clemson, SC 29630, USACurio Wellness, P.O. Box 5470, Towson, MD 21285, USAPlant and Environmental Science Department, 171 Poole Agricultural Center, Clemson University, Clemson, SC 29630, USAIn vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with <i>Cannabis sativa</i> (‘Cherry1’, ‘BaOx’, ‘T1’, ‘Peach’) grown in vessels of three different physical states—stationary agar (A); stationary Oasis<sup>®</sup> infused with liquid (OILs); and agitated Oasis<sup>®</sup> infused with liquid (OILa). Vessels were pre-selected as control or supplemented; where supplement vessels received 15 mL DKW liquid media each cycle harvest. The number of shoot tips harvested; shoot length; and dry shoot mass from repeated cutting cycles was recorded. In a single harvest; ‘BaOx’ and ‘Cherry 1’ produced one shoot per plant from the original 15 planted on all treatments. ‘Peach’ and ‘T1’ produced less shoots on average; but the most in OIL treatments. All shoots harvested were longer in OIL compared to A; regardless of genotype. Over multiple cycles; ‘Peach’ and ‘T1’ were unable to reliably produce shoots on a repeated schedule and were, therefore, eliminated from the experiment. By cycle 3; maximum number of plants were produced; regardless of supplementation (‘Cherry 1’; 30; ‘BaOx’; 22). Shoot length was above 10 mm (planting standard) for both genotypes until after the third cycle (10 weeks) where number and quality decreased (nodes and internodes easily discerned). By the end of the experiment; the only shoots that remained productive for over 16 weeks and multiple repeated harvest cycles were those in OIL treatments with supplements.https://www.mdpi.com/2223-7747/14/7/1138micropropagationhedgingfed-batchcannabistissue culturecytokinin |
| spellingShingle | Molly McKay James E. Faust Matthew Taylor Jeffrey Adelberg Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin Plants micropropagation hedging fed-batch cannabis tissue culture cytokinin |
| title | Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin |
| title_full | Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin |
| title_fullStr | Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin |
| title_full_unstemmed | Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin |
| title_short | Modified Media for Repeated In Vitro Cutting Cycles of <i>Cannabis sativa</i> Without the Use of Cytokinin |
| title_sort | modified media for repeated in vitro cutting cycles of i cannabis sativa i without the use of cytokinin |
| topic | micropropagation hedging fed-batch cannabis tissue culture cytokinin |
| url | https://www.mdpi.com/2223-7747/14/7/1138 |
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