Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits
We evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/270253 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849403265995767808 |
|---|---|
| author | Jennifher Góngora Andrea Díaz-Roa Alejandro Ramírez-Hernández Jesús A. Cortés-Vecino María A. Gaona Manuel A. Patarroyo Felio Bello |
| author_facet | Jennifher Góngora Andrea Díaz-Roa Alejandro Ramírez-Hernández Jesús A. Cortés-Vecino María A. Gaona Manuel A. Patarroyo Felio Bello |
| author_sort | Jennifher Góngora |
| collection | DOAJ |
| description | We evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected with Staphylococcus aureus and Pseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies’ larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken from S. magellanica had better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species’ fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds. |
| format | Article |
| id | doaj-art-900a8127d1c94c74bbcb8cbe1b96fe82 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-900a8127d1c94c74bbcb8cbe1b96fe822025-08-20T03:37:19ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/270253270253Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic RabbitsJennifher Góngora0Andrea Díaz-Roa1Alejandro Ramírez-Hernández2Jesús A. Cortés-Vecino3María A. Gaona4Manuel A. Patarroyo5Felio Bello6Medical and Forensic Entomology Laboratory, School of Medicine and Health Sciences, Universidad del Rosario, Carrera 24 No. 63C-69, Bogotá, ColombiaMedical and Forensic Entomology Laboratory, School of Medicine and Health Sciences, Universidad del Rosario, Carrera 24 No. 63C-69, Bogotá, ColombiaVeterinary Parasitology Laboratory, Faculty of Veterinary Medicine and Animal Husbandry, Universidad Nacional de Colombia, Carrera 45 No. 26-85, Bogotá, ColombiaVeterinary Parasitology Laboratory, Faculty of Veterinary Medicine and Animal Husbandry, Universidad Nacional de Colombia, Carrera 45 No. 26-85, Bogotá, ColombiaMicrobiology Laboratory, Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Carrera 24 No. 63C-69, Bogotá, ColombiaMolecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia, Avenida 50 No. 26-20, Bogotá, ColombiaMedical and Forensic Entomology Laboratory, School of Medicine and Health Sciences, Universidad del Rosario, Carrera 24 No. 63C-69, Bogotá, ColombiaWe evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected with Staphylococcus aureus and Pseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies’ larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken from S. magellanica had better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species’ fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds.http://dx.doi.org/10.1155/2015/270253 |
| spellingShingle | Jennifher Góngora Andrea Díaz-Roa Alejandro Ramírez-Hernández Jesús A. Cortés-Vecino María A. Gaona Manuel A. Patarroyo Felio Bello Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits Journal of Diabetes Research |
| title | Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits |
| title_full | Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits |
| title_fullStr | Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits |
| title_full_unstemmed | Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits |
| title_short | Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits |
| title_sort | evaluating the effect of sarconesiopsis magellanica diptera calliphoridae larvae derived haemolymph and fat body extracts on chronic wounds in diabetic rabbits |
| url | http://dx.doi.org/10.1155/2015/270253 |
| work_keys_str_mv | AT jennifhergongora evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT andreadiazroa evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT alejandroramirezhernandez evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT jesusacortesvecino evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT mariaagaona evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT manuelapatarroyo evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits AT feliobello evaluatingtheeffectofsarconesiopsismagellanicadipteracalliphoridaelarvaederivedhaemolymphandfatbodyextractsonchronicwoundsindiabeticrabbits |