Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed
Fenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplement ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore,...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2020-0013 |
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| author | Król-Kogus Barbara Głód Daniel Krauze-Baranowska Mirosława |
| author_facet | Król-Kogus Barbara Głód Daniel Krauze-Baranowska Mirosława |
| author_sort | Król-Kogus Barbara |
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| description | Fenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplement ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSDESI-MS method. Two C-18 columns connected in series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminarily validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %. |
| format | Article |
| id | doaj-art-8fffd576a4134637b9ba5b6308335136 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-8fffd576a4134637b9ba5b63083351362025-02-03T08:57:08ZengSciendoActa Pharmaceutica1846-95582020-03-01701899910.2478/acph-2020-0013acph-2020-0013Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seedKról-Kogus Barbara0Głód Daniel1Krauze-Baranowska Mirosława2Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416Gdańsk, PolandFenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplement ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSDESI-MS method. Two C-18 columns connected in series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminarily validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %.https://doi.org/10.2478/acph-2020-0013fenugreek seedssaponinshplc-elsd-esi-msqualitative and quantitative analysis |
| spellingShingle | Król-Kogus Barbara Głód Daniel Krauze-Baranowska Mirosława Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed Acta Pharmaceutica fenugreek seeds saponins hplc-elsd-esi-ms qualitative and quantitative analysis |
| title | Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed |
| title_full | Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed |
| title_fullStr | Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed |
| title_full_unstemmed | Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed |
| title_short | Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed |
| title_sort | qualitative and quantitative hplc elsd esi ms analysis of steroidal saponins in fenugreek seed |
| topic | fenugreek seeds saponins hplc-elsd-esi-ms qualitative and quantitative analysis |
| url | https://doi.org/10.2478/acph-2020-0013 |
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