Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice
The purinergic P2X7 receptor is expressed by bone cells and has been shown to be important in both bone formation and bone resorption. In this study we investigated the importance of the genetic background of the mouse strains on which the P2X7 knock-out models were based by comparing bone status of...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Osteoporosis |
| Online Access: | http://dx.doi.org/10.1155/2012/391097 |
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| author | Susanne Syberg Solveig Petersen Jens-Erik Beck Jensen Alison Gartland Jenni Teilmann Iain Chessell Thomas H. Steinberg Peter Schwarz Niklas Rye Jørgensen |
| author_facet | Susanne Syberg Solveig Petersen Jens-Erik Beck Jensen Alison Gartland Jenni Teilmann Iain Chessell Thomas H. Steinberg Peter Schwarz Niklas Rye Jørgensen |
| author_sort | Susanne Syberg |
| collection | DOAJ |
| description | The purinergic P2X7 receptor is expressed by bone cells and has been shown to be important in both bone formation and bone resorption. In this study we investigated the importance of the genetic background of the mouse strains on which the P2X7 knock-out models were based by comparing bone status of a new BALB/cJ P2X7−/− strain with a previous one based on the C57BL/6 strain. Female four-month-old mice from both strains were DXA scanned on a PIXImus densitometer; femurs were collected for bone strength measurements and serum for bone marker analysis. Bone-related parameters that were altered only slightly in the B6 P2X7−/− became significantly altered in the BALB/cJ P2X7−/− when compared to their wild type littermates. The BALB/cJ P2X7−/− showed reduced levels of serum C-telopeptide fragment (s-CTX), higher bone mineral density, and increased bone strength compared to the wild type littermates. In conclusion, we have shown that the genetic background of P2X7−/− mice strongly influences the bone phenotype of the P2X7−/− mice and that P2X7 has a more significant regulatory role in bone remodeling than found in previous studies. |
| format | Article |
| id | doaj-art-8ff3cad7aa064479925ea51e018b83f2 |
| institution | Kabale University |
| issn | 2090-8059 2042-0064 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Osteoporosis |
| spelling | doaj-art-8ff3cad7aa064479925ea51e018b83f22025-02-03T01:01:56ZengWileyJournal of Osteoporosis2090-80592042-00642012-01-01201210.1155/2012/391097391097Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout MiceSusanne Syberg0Solveig Petersen1Jens-Erik Beck Jensen2Alison Gartland3Jenni Teilmann4Iain Chessell5Thomas H. Steinberg6Peter Schwarz7Niklas Rye Jørgensen8Departments of Diagnostics and Medicine, Research Centre of Ageing and Osteoporosis, Glostrup University Hospital, 2600 Glostrup, DenmarkDepartments of Diagnostics and Medicine, Research Centre of Ageing and Osteoporosis, Glostrup University Hospital, 2600 Glostrup, DenmarkOsteoporosis and Bone Metabolic Unit, Departments of Endocrinology and Clinical Biochemistry, Hvidovre University Hospital, 2650 Hvidovre, DenmarkMellanby Centre for Bone Research, The University of Sheffield, Sheffield S10 2TN, UKOsteoporosis and Bone Metabolic Unit, Departments of Endocrinology and Clinical Biochemistry, Hvidovre University Hospital, 2650 Hvidovre, DenmarkNeuroScience and MedImmune, Milstein Building, Granta Park, Cambridge CB21 6GH, UKDepartment of Internal Medicine, Washington University School of Medicine and St. Louis Veterans Affairs Medical Center, St. Louis, MO 63106, USADepartments of Diagnostics and Medicine, Research Centre of Ageing and Osteoporosis, Glostrup University Hospital, 2600 Glostrup, DenmarkDepartments of Diagnostics and Medicine, Research Centre of Ageing and Osteoporosis, Glostrup University Hospital, 2600 Glostrup, DenmarkThe purinergic P2X7 receptor is expressed by bone cells and has been shown to be important in both bone formation and bone resorption. In this study we investigated the importance of the genetic background of the mouse strains on which the P2X7 knock-out models were based by comparing bone status of a new BALB/cJ P2X7−/− strain with a previous one based on the C57BL/6 strain. Female four-month-old mice from both strains were DXA scanned on a PIXImus densitometer; femurs were collected for bone strength measurements and serum for bone marker analysis. Bone-related parameters that were altered only slightly in the B6 P2X7−/− became significantly altered in the BALB/cJ P2X7−/− when compared to their wild type littermates. The BALB/cJ P2X7−/− showed reduced levels of serum C-telopeptide fragment (s-CTX), higher bone mineral density, and increased bone strength compared to the wild type littermates. In conclusion, we have shown that the genetic background of P2X7−/− mice strongly influences the bone phenotype of the P2X7−/− mice and that P2X7 has a more significant regulatory role in bone remodeling than found in previous studies.http://dx.doi.org/10.1155/2012/391097 |
| spellingShingle | Susanne Syberg Solveig Petersen Jens-Erik Beck Jensen Alison Gartland Jenni Teilmann Iain Chessell Thomas H. Steinberg Peter Schwarz Niklas Rye Jørgensen Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice Journal of Osteoporosis |
| title | Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice |
| title_full | Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice |
| title_fullStr | Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice |
| title_full_unstemmed | Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice |
| title_short | Genetic Background Strongly Influences the Bone Phenotype of P2X7 Receptor Knockout Mice |
| title_sort | genetic background strongly influences the bone phenotype of p2x7 receptor knockout mice |
| url | http://dx.doi.org/10.1155/2012/391097 |
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