Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta
Colocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties w...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2021-10-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Tumor-Anti-Initiation-and-Anti-Progression-Properties-of-Sulphated-Extract-of-Colocasia,142835,0,2.html |
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| author | Amira M. Gamal-Eldeen Hassan Amer Cinderella A. Fahmy Haytham Dahlawi Basem H Elesawy Nahla L. Faizo Bassem M. Raafat |
| author_facet | Amira M. Gamal-Eldeen Hassan Amer Cinderella A. Fahmy Haytham Dahlawi Basem H Elesawy Nahla L. Faizo Bassem M. Raafat |
| author_sort | Amira M. Gamal-Eldeen |
| collection | DOAJ |
| description | Colocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties were explored. The amending of carcinogen metabolism and radical scavenging affinity revealed that SCE is a strong tumor anti-initiation agent via suppressing cytochrome P450-1A and enhancing glutathione and the carcinogen detoxification enzyme; glutathione S-transferase. SCE exhibited a strong scavenging affinity towards critical radicals (hydroxyl and peroxyl). It induced lymphocyte growth and modulated the macrophage functions into an anti-inflammatory profile, via elevating macrophage proliferation and its binding affinity of fluorescein isothiocyanate-lipopolysaccharide (FITC-LPS) and inhibiting nitric oxide and tumor necrosis factor-α generation. Furthermore, SCE showed a potent cytotoxicity against human breast MCF-7 carcinoma cells (IC 50 27.73 µg/mL), whereas SCE treatment inhibited the activity of histone deacetylase (HDAC IC 50 37.70 µg/mL) and disturbed the pattern of cell cycle phases. An arrest in both S- and G2/M-phases was linked with shifted cell populations towards late apoptosis and necrosis, as detected by flow cytometry. SCE is a promising cancer chemopreventive agent to be used in healthy food industries and for the high breast cancer-risk population. |
| format | Article |
| id | doaj-art-8fea45148b55471593d312f45a889578 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-8fea45148b55471593d312f45a8895782025-02-02T01:53:57ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072021-10-0171439340110.31883/pjfns/142835142835Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculentaAmira M. Gamal-Eldeen0Hassan Amer 1https://orcid.org/0000-0002-9595-1102Cinderella A. Fahmy2https://orcid.org/0000-0002-2629-2473Haytham Dahlawi3https://orcid.org/0000-0002-4406-4554Basem H Elesawy4https://orcid.org/0000-0002-2581-265XNahla L. Faizo5https://orcid.org/0000-0003-4451-7602Bassem M. Raafat6https://orcid.org/0000-0003-4612-8811Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo 12622, EgyptCancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, EgyptClinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaRadiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaRadiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaColocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties were explored. The amending of carcinogen metabolism and radical scavenging affinity revealed that SCE is a strong tumor anti-initiation agent via suppressing cytochrome P450-1A and enhancing glutathione and the carcinogen detoxification enzyme; glutathione S-transferase. SCE exhibited a strong scavenging affinity towards critical radicals (hydroxyl and peroxyl). It induced lymphocyte growth and modulated the macrophage functions into an anti-inflammatory profile, via elevating macrophage proliferation and its binding affinity of fluorescein isothiocyanate-lipopolysaccharide (FITC-LPS) and inhibiting nitric oxide and tumor necrosis factor-α generation. Furthermore, SCE showed a potent cytotoxicity against human breast MCF-7 carcinoma cells (IC 50 27.73 µg/mL), whereas SCE treatment inhibited the activity of histone deacetylase (HDAC IC 50 37.70 µg/mL) and disturbed the pattern of cell cycle phases. An arrest in both S- and G2/M-phases was linked with shifted cell populations towards late apoptosis and necrosis, as detected by flow cytometry. SCE is a promising cancer chemopreventive agent to be used in healthy food industries and for the high breast cancer-risk population.http://journal.pan.olsztyn.pl/Tumor-Anti-Initiation-and-Anti-Progression-Properties-of-Sulphated-Extract-of-Colocasia,142835,0,2.htmlcolocasia esculentacyp1acancer chemoprevention & tumor anti-initiatinghdacmacrophage functionbreast mcf-7 carcinoma |
| spellingShingle | Amira M. Gamal-Eldeen Hassan Amer Cinderella A. Fahmy Haytham Dahlawi Basem H Elesawy Nahla L. Faizo Bassem M. Raafat Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta Polish Journal of Food and Nutrition Sciences colocasia esculenta cyp1a cancer chemoprevention & tumor anti-initiating hdac macrophage function breast mcf-7 carcinoma |
| title | Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta |
| title_full | Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta |
| title_fullStr | Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta |
| title_full_unstemmed | Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta |
| title_short | Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta |
| title_sort | tumor anti initiation and anti progression properties of sulphated extract of colocasia esculenta |
| topic | colocasia esculenta cyp1a cancer chemoprevention & tumor anti-initiating hdac macrophage function breast mcf-7 carcinoma |
| url | http://journal.pan.olsztyn.pl/Tumor-Anti-Initiation-and-Anti-Progression-Properties-of-Sulphated-Extract-of-Colocasia,142835,0,2.html |
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