Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method
An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/292754 |
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| author | V. Bhaskara Raju A. Lakshmana Rao |
| author_facet | V. Bhaskara Raju A. Lakshmana Rao |
| author_sort | V. Bhaskara Raju |
| collection | DOAJ |
| description | An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μ
g/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms. |
| format | Article |
| id | doaj-art-8fe83d922b2541159625073c0cab8599 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-8fe83d922b2541159625073c0cab85992025-02-03T06:13:40ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019134034410.1155/2012/292754Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC MethodV. Bhaskara Raju0A. Lakshmana Rao1Sri Vasavi Institute of Pharmaceutical Sciences, Tadepalligudem- 534 101, A.P., IndiaV.V. Institute of Pharmaceutical Sciences, Gudlavalleru-521 356, A.P., IndiaAn accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μ g/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.http://dx.doi.org/10.1155/2012/292754 |
| spellingShingle | V. Bhaskara Raju A. Lakshmana Rao Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method E-Journal of Chemistry |
| title | Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method |
| title_full | Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method |
| title_fullStr | Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method |
| title_full_unstemmed | Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method |
| title_short | Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method |
| title_sort | development estimation and validation of lisinopril in bulk and its pharmaceutical formulation by hplc method |
| url | http://dx.doi.org/10.1155/2012/292754 |
| work_keys_str_mv | AT vbhaskararaju developmentestimationandvalidationoflisinoprilinbulkanditspharmaceuticalformulationbyhplcmethod AT alakshmanarao developmentestimationandvalidationoflisinoprilinbulkanditspharmaceuticalformulationbyhplcmethod |