X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors
Contrast agents that specifically enhance cancers on magnetic resonance imaging (MRI) will allow earlier detection. Vanadium-based chelates (VCs) selectively enhance rodent cancers on MRI, suggesting selective uptake of VCs by cancers. Here we report x-ray fluorescence microscopy (XFM) of VC uptake...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2015-05-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2015.00001 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841564474913325056 |
|---|---|
| author | Devkumar Mustafi Jesse Ward Urszula Dougherty Marc Bissonnette John Hart Stefan Vogt Gregory S. Karczmar |
| author_facet | Devkumar Mustafi Jesse Ward Urszula Dougherty Marc Bissonnette John Hart Stefan Vogt Gregory S. Karczmar |
| author_sort | Devkumar Mustafi |
| collection | DOAJ |
| description | Contrast agents that specifically enhance cancers on magnetic resonance imaging (MRI) will allow earlier detection. Vanadium-based chelates (VCs) selectively enhance rodent cancers on MRI, suggesting selective uptake of VCs by cancers. Here we report x-ray fluorescence microscopy (XFM) of VC uptake by murine colon cancer. Colonic tumors in mice treated with azoxymethane/dextran sulfate sodium were identified by MRI. Then a gadolinium-based contrast agent and a VC were injected intravenously; mice were sacrificed and colons sectioned. VC distribution was sampled at 120 minutes after injection to evaluate the long-term accumulation. Gadolinium distribution was sampled at 10 minutes after injection due to its rapid washout. XFM was performed on 72 regions of normal and cancerous colon from five normal mice and four cancer-bearing mice. XFM showed that all gadolinium was extracellular, with similar concentrations in colon cancers and normal colon. In contrast, the average VC concentration was twofold higher in cancers versus normal tissue ( p < .002). Cancers also contained numerous “hot spots” with intracellular VC concentrations sixfold higher than the concentration in normal colon ( p < .0001). No hot spots were detected in normal colon. This is the first direct demonstration that VCs selectively accumulate in cancer cells and thus may improve cancer detection. |
| format | Article |
| id | doaj-art-8fe2ca56e4b34f7fbd6a3b7385b2a339 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-8fe2ca56e4b34f7fbd6a3b7385b2a3392025-01-02T22:38:22ZengSAGE PublishingMolecular Imaging1536-01212015-05-011410.2310/7290.2015.0000110.2310_7290.2015.00001X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic TumorsDevkumar MustafiJesse WardUrszula DoughertyMarc BissonnetteJohn HartStefan VogtGregory S. KarczmarContrast agents that specifically enhance cancers on magnetic resonance imaging (MRI) will allow earlier detection. Vanadium-based chelates (VCs) selectively enhance rodent cancers on MRI, suggesting selective uptake of VCs by cancers. Here we report x-ray fluorescence microscopy (XFM) of VC uptake by murine colon cancer. Colonic tumors in mice treated with azoxymethane/dextran sulfate sodium were identified by MRI. Then a gadolinium-based contrast agent and a VC were injected intravenously; mice were sacrificed and colons sectioned. VC distribution was sampled at 120 minutes after injection to evaluate the long-term accumulation. Gadolinium distribution was sampled at 10 minutes after injection due to its rapid washout. XFM was performed on 72 regions of normal and cancerous colon from five normal mice and four cancer-bearing mice. XFM showed that all gadolinium was extracellular, with similar concentrations in colon cancers and normal colon. In contrast, the average VC concentration was twofold higher in cancers versus normal tissue ( p < .002). Cancers also contained numerous “hot spots” with intracellular VC concentrations sixfold higher than the concentration in normal colon ( p < .0001). No hot spots were detected in normal colon. This is the first direct demonstration that VCs selectively accumulate in cancer cells and thus may improve cancer detection.https://doi.org/10.2310/7290.2015.00001 |
| spellingShingle | Devkumar Mustafi Jesse Ward Urszula Dougherty Marc Bissonnette John Hart Stefan Vogt Gregory S. Karczmar X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors Molecular Imaging |
| title | X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors |
| title_full | X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors |
| title_fullStr | X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors |
| title_full_unstemmed | X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors |
| title_short | X-Ray Fluorescence Microscopy Demonstrates Preferential Accumulation of a Vanadium-Based Magnetic Resonance Imaging Contrast Agent in Murine Colonic Tumors |
| title_sort | x ray fluorescence microscopy demonstrates preferential accumulation of a vanadium based magnetic resonance imaging contrast agent in murine colonic tumors |
| url | https://doi.org/10.2310/7290.2015.00001 |
| work_keys_str_mv | AT devkumarmustafi xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT jesseward xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT urszuladougherty xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT marcbissonnette xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT johnhart xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT stefanvogt xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors AT gregoryskarczmar xrayfluorescencemicroscopydemonstratespreferentialaccumulationofavanadiumbasedmagneticresonanceimagingcontrastagentinmurinecolonictumors |