Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)

The genetic wealth of range-restricted species is threatened by human activities such as habitat fragmentation or alteration. This research focused on the genotype effect in sexual propagation with freshly collected seeds of vulnerable local endemic plants in Crete, namely, <i>Campanula pelvif...

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Main Authors: Ioannis Anestis, Elias Pipinis, Eleftherios Karapatzak, Stefanos Kostas, Georgios Menexes, Eleftherios Dariotis, Georgios Tsoktouridis, Stefanos Hatzilazarou, Nikos Krigas
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Language:English
Published: MDPI AG 2024-10-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/10/11/1149
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author Ioannis Anestis
Elias Pipinis
Eleftherios Karapatzak
Stefanos Kostas
Georgios Menexes
Eleftherios Dariotis
Georgios Tsoktouridis
Stefanos Hatzilazarou
Nikos Krigas
author_facet Ioannis Anestis
Elias Pipinis
Eleftherios Karapatzak
Stefanos Kostas
Georgios Menexes
Eleftherios Dariotis
Georgios Tsoktouridis
Stefanos Hatzilazarou
Nikos Krigas
author_sort Ioannis Anestis
collection DOAJ
description The genetic wealth of range-restricted species is threatened by human activities such as habitat fragmentation or alteration. This research focused on the genotype effect in sexual propagation with freshly collected seeds of vulnerable local endemic plants in Crete, namely, <i>Campanula pelviformis</i> and <i>Petromarula pinnata</i> (Campanulaceae), which can be sustainably utilized for agro-alimentary, medicinal, and ornamental purposes. The morphological characteristics of seed length, width, and weight of freshly collected seeds from five wild-growing populations of <i>C. pelviformis</i> and seven wild-growing populations of <i>P. pinnata</i> from lowlands (<500 m) and semi-mountainous (>500 m) altitudes were measured and subjected to germination experiments at four incubation temperature treatments (10, 15, 20, and 25 °C). The effects of temperature, genotype, and their interactions were statistically significant for seed germination, with the results similar to those of previous studies on stored seeds of the same populations. Freshly collected seeds of both species germinated best at incubation temperatures of 10 °C and 15 °C (>68.13% for all population of <i>C. pelviformis</i> and >80.63% for six out of seven populations of <i>P. pinnata</i>), while significant differences were detected among genotypes for both species at each incubation temperature and among genotypes, even from similar altitudes. A statistically significant effect of altitude on germination percentages was observed at an incubation temperature of 20 °C between lowland and semi-mountainous genotypes for both species. The data produced in this study may enhance conservation efforts and lead to species-specific information that can be exploited in the future for their introduction as novel multi-purpose crops.
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spelling doaj-art-8fe2bb2592994b26b991a58694c3bc722025-08-20T01:53:56ZengMDPI AGHorticulturae2311-75242024-10-011011114910.3390/horticulturae10111149Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)Ioannis Anestis0Elias Pipinis1Eleftherios Karapatzak2Stefanos Kostas3Georgios Menexes4Eleftherios Dariotis5Georgios Tsoktouridis6Stefanos Hatzilazarou7Nikos Krigas8Laboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Silviculture, School of Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thermi, GreeceLaboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Agronomy, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thermi, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thermi, GreeceLaboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thermi, GreeceThe genetic wealth of range-restricted species is threatened by human activities such as habitat fragmentation or alteration. This research focused on the genotype effect in sexual propagation with freshly collected seeds of vulnerable local endemic plants in Crete, namely, <i>Campanula pelviformis</i> and <i>Petromarula pinnata</i> (Campanulaceae), which can be sustainably utilized for agro-alimentary, medicinal, and ornamental purposes. The morphological characteristics of seed length, width, and weight of freshly collected seeds from five wild-growing populations of <i>C. pelviformis</i> and seven wild-growing populations of <i>P. pinnata</i> from lowlands (<500 m) and semi-mountainous (>500 m) altitudes were measured and subjected to germination experiments at four incubation temperature treatments (10, 15, 20, and 25 °C). The effects of temperature, genotype, and their interactions were statistically significant for seed germination, with the results similar to those of previous studies on stored seeds of the same populations. Freshly collected seeds of both species germinated best at incubation temperatures of 10 °C and 15 °C (>68.13% for all population of <i>C. pelviformis</i> and >80.63% for six out of seven populations of <i>P. pinnata</i>), while significant differences were detected among genotypes for both species at each incubation temperature and among genotypes, even from similar altitudes. A statistically significant effect of altitude on germination percentages was observed at an incubation temperature of 20 °C between lowland and semi-mountainous genotypes for both species. The data produced in this study may enhance conservation efforts and lead to species-specific information that can be exploited in the future for their introduction as novel multi-purpose crops.https://www.mdpi.com/2311-7524/10/11/1149biodiversityseed germinationneglected and underutilized plantsagro-alimentarymedicinalornamental
spellingShingle Ioannis Anestis
Elias Pipinis
Eleftherios Karapatzak
Stefanos Kostas
Georgios Menexes
Eleftherios Dariotis
Georgios Tsoktouridis
Stefanos Hatzilazarou
Nikos Krigas
Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
Horticulturae
biodiversity
seed germination
neglected and underutilized plants
agro-alimentary
medicinal
ornamental
title Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
title_full Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
title_fullStr Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
title_full_unstemmed Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
title_short Effect of Genotype and Altitude on the Germination of Freshly Collected Seeds from Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and <i>Petromarula pinnata</i> (L.) A. DC. (Campanulaceae)
title_sort effect of genotype and altitude on the germination of freshly collected seeds from wild growing populations of i campanula pelviformis i lam and i petromarula pinnata i l a dc campanulaceae
topic biodiversity
seed germination
neglected and underutilized plants
agro-alimentary
medicinal
ornamental
url https://www.mdpi.com/2311-7524/10/11/1149
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