Protein/Amphipathic Polyamine Complexes Enable Highly Efficient Transfection with Minimal Toxicity

Ternary complexes of plasmid DNA, histone H1 protein and amphipathic polyamines (PAPA) were able to mediate the efficient transfection of 3T3, HeLa and COS cells in culture. Using both the β-galactosidase and luciferase reporter gene systems, the transfection efficiency of PAPA complexes was compara...

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Bibliographic Details
Main Authors: V. Budker, J.E. Hagstrom, O. Lapina, D. Eifrig, J. Fritz, J.A. Wolff
Format: Article
Language:English
Published: Taylor & Francis Group 1997-07-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97231rr02
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