Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro

Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion (AMX-LH-NE) against three main pathogenic bacteria of cow mastitis in vitro was investigated by single drug bridging test, compound drug susceptibility test, single drug minimum inhibitory concentrat...

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Main Authors: YANG Xue-feng, OUYANG Wu-qing, LI Xiang-hui, ZHANG Wen-juan, LIU Yu-mei
Format: Article
Language:English
Published: Zhejiang University Press 2009-09-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.017
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author YANG Xue-feng
OUYANG Wu-qing
LI Xiang-hui
ZHANG Wen-juan
LIU Yu-mei
author_facet YANG Xue-feng
OUYANG Wu-qing
LI Xiang-hui
ZHANG Wen-juan
LIU Yu-mei
author_sort YANG Xue-feng
collection DOAJ
description Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion (AMX-LH-NE) against three main pathogenic bacteria of cow mastitis in vitro was investigated by single drug bridging test, compound drug susceptibility test, single drug minimum inhibitory concentration (MIC) determination, combination susceptibility test and Kirby-Baueer disc diffusion test. The results show that synergistic effect was generated when amoxicillin (AMX) and levofloxacin hydrochloride (LH) were combined. MIC of the mixed AMX and LH against Staphylococcus aureus was 1/4 and 1/8 times compared with single drug, respectively, and MIC against Streptococcus agalactiae was both 1/4 times, and MIC against Escherichia coli was both 1/8 times. Each of fractional inhibitory concentration (FIC) index was not more than 0.5. The disc diffusion zone diameters of AMX-LH-NE against bacteria were all highly significant difference (P<0.01) compared with other each group. The results above suggested that combination antibacterial activity of AMX-LH-NE against three pathogenic bacteria was strong.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-8fde553532084975b3d7c974effe08a92025-08-20T02:54:53ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-09-013558559010.3785/j.issn.1008-9209.2009.05.01710089209Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitroYANG Xue-fengOUYANG Wu-qingLI Xiang-huiZHANG Wen-juanLIU Yu-meiCombination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion (AMX-LH-NE) against three main pathogenic bacteria of cow mastitis in vitro was investigated by single drug bridging test, compound drug susceptibility test, single drug minimum inhibitory concentration (MIC) determination, combination susceptibility test and Kirby-Baueer disc diffusion test. The results show that synergistic effect was generated when amoxicillin (AMX) and levofloxacin hydrochloride (LH) were combined. MIC of the mixed AMX and LH against Staphylococcus aureus was 1/4 and 1/8 times compared with single drug, respectively, and MIC against Streptococcus agalactiae was both 1/4 times, and MIC against Escherichia coli was both 1/8 times. Each of fractional inhibitory concentration (FIC) index was not more than 0.5. The disc diffusion zone diameters of AMX-LH-NE against bacteria were all highly significant difference (P&lt;0.01) compared with other each group. The results above suggested that combination antibacterial activity of AMX-LH-NE against three pathogenic bacteria was strong.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.017amoxicillinlevofloxacin hydrochloridenanoemulsion<italic>Staphylococcus aureus</italic> <italic>Streptococcus agalactiae</italic> <italic>Escherichia coli</italic> synergistic effect
spellingShingle YANG Xue-feng
OUYANG Wu-qing
LI Xiang-hui
ZHANG Wen-juan
LIU Yu-mei
Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
浙江大学学报. 农业与生命科学版
amoxicillin
levofloxacin hydrochloride
nanoemulsion
<italic>Staphylococcus aureus</italic>
<italic>Streptococcus agalactiae</italic>
<italic>Escherichia coli</italic>
synergistic effect
title Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
title_full Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
title_fullStr Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
title_full_unstemmed Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
title_short Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
title_sort combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro
topic amoxicillin
levofloxacin hydrochloride
nanoemulsion
<italic>Staphylococcus aureus</italic>
<italic>Streptococcus agalactiae</italic>
<italic>Escherichia coli</italic>
synergistic effect
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.017
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