GmTRAB1, a Basic Leucine Zipper Transcription Factor, Positively Regulates Drought Tolerance in Soybean (<i>Glycine max</i>. L)

The basic leucine zipper (bZIP) transcription factors play crucial roles in plant resistance to environmental challenges, but the biological functions of soybean bZIP members are still unclear. In this study, a drought-related soybean bZIP gene, <i>GmTRAB</i>1, was analyzed. The transcri...

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Main Authors: Hui Li, Qiu-Yu Zhang, Ping Xu, Xiao-Hua Wang, Sheng-Jie Dai, Zhen-Ning Liu, Meng Xu, Xue Cao, Xiao-Yu Cui
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/21/3104
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Summary:The basic leucine zipper (bZIP) transcription factors play crucial roles in plant resistance to environmental challenges, but the biological functions of soybean bZIP members are still unclear. In this study, a drought-related soybean bZIP gene, <i>GmTRAB</i>1, was analyzed. The transcript of <i>GmTRAB1</i> was upregulated under drought, ABA, and oxidative stresses. Overexpression of <i>GmTRAB1</i> improved the osmotic stress tolerance of transgenic <i>Arabidopsis</i> and soybean hairy roots associated with increased proline content and activity of antioxidant enzymes and reduced accumulations of malonaldehyde and reactive oxide species. However, RNA interference silencing of <i>GmTRAB1</i> in the soybean hairy roots improved drought sensitivity. Furthermore, GmTRAB1 increased the sensitivity of transgenic plants to ABA and participated in modulating ABA-regulated stomatal closure upon drought stress. In addition, GmTRAB1 stimulated the transcript accumulation of drought-, ABA-, and antioxidant-related genes to respond to drought. Collectively, this research will contribute to understanding the molecular mechanisms of bZIP transcription factors in soybean’s resistance to drought.
ISSN:2223-7747