Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal

Citrus fruit juice is highly beneficial to human health, providing essential nutrients like carbohydrates, vitamins, phytochemicals, and antioxidants. Juices from citrus fruit varieties grown in various regions of Nepal were analyzed for their physicochemical properties, nutraceutical content, antio...

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Main Authors: Nirmal Karki, Hari Achhami, Bishwa Bandhu Pachhai, Susmita Bhattarai, Dikpal Kumar Shahi, Lok Ranjan Bhatt, Mahesh Kumar Joshi
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024168041
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author Nirmal Karki
Hari Achhami
Bishwa Bandhu Pachhai
Susmita Bhattarai
Dikpal Kumar Shahi
Lok Ranjan Bhatt
Mahesh Kumar Joshi
author_facet Nirmal Karki
Hari Achhami
Bishwa Bandhu Pachhai
Susmita Bhattarai
Dikpal Kumar Shahi
Lok Ranjan Bhatt
Mahesh Kumar Joshi
author_sort Nirmal Karki
collection DOAJ
description Citrus fruit juice is highly beneficial to human health, providing essential nutrients like carbohydrates, vitamins, phytochemicals, and antioxidants. Juices from citrus fruit varieties grown in various regions of Nepal were analyzed for their physicochemical properties, nutraceutical content, antioxidant and antimicrobial properties. The pH of the juices ranged between 2.09 and 3.62, while total soluble solids (TSS) varied from 7° Brix to 12.3 °Brix. Among varieties, C. aurantifolia exhibited the highest titratable acidity at 7.39 g/100 mL. C. limon showed the highest moisture content (94.74 %), C. reticulata had the highest carbohydrate content (14.6 ± 0.4 g/100 mL, n = 3), and C. aurantifolia presented the highest protein content (34.1 ± 0.7 mg/100 mL). C. sinensis recorded the highest flavonoid content (91.4 ± 0.3 mg/100 mL), C. reticulata had the highest phenolic content (65.8 ± 0.6 mg/100 mL), and C. limon exhibited the highest ascorbic acid content (45.1 ± 0.4 mg/100 mL). The methanolic extracts of all citrus fruit juices demonstrated robust antioxidant properties, as determined by DPPH assay. Notably, C. limon and C. aurantifolia juice extracts demonstrated significant antimicrobial activity against a wide range of microbes. This study highlights the variation in nutrient and phytochemicals compositions among different citrus fruit juices, underscoring the nutritional and medicinal benefits of citrus species.
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spelling doaj-art-8fcc1c2060354b6c94e4b10ea07083eb2025-08-20T01:59:38ZengElsevierHeliyon2405-84402024-12-011023e4077310.1016/j.heliyon.2024.e40773Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from NepalNirmal Karki0Hari Achhami1Bishwa Bandhu Pachhai2Susmita Bhattarai3Dikpal Kumar Shahi4Lok Ranjan Bhatt5Mahesh Kumar Joshi6Department of Chemistry, Trichandra Multiple Campus, Tribhuvan University, Kathmandu, NepalBiological Resources Unit, Faculty of Science, Nepal Academy of Science & Technology, Khumaltar, Lalitpur, 44700, NepalBiological Resources Unit, Faculty of Science, Nepal Academy of Science & Technology, Khumaltar, Lalitpur, 44700, NepalDepartment of Chemistry, Trichandra Multiple Campus, Tribhuvan University, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NepalBiological Resources Unit, Faculty of Science, Nepal Academy of Science & Technology, Khumaltar, Lalitpur, 44700, Nepal; Corresponding author.Department of Chemistry, Trichandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal; Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal; Corresponding author. Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal.Citrus fruit juice is highly beneficial to human health, providing essential nutrients like carbohydrates, vitamins, phytochemicals, and antioxidants. Juices from citrus fruit varieties grown in various regions of Nepal were analyzed for their physicochemical properties, nutraceutical content, antioxidant and antimicrobial properties. The pH of the juices ranged between 2.09 and 3.62, while total soluble solids (TSS) varied from 7° Brix to 12.3 °Brix. Among varieties, C. aurantifolia exhibited the highest titratable acidity at 7.39 g/100 mL. C. limon showed the highest moisture content (94.74 %), C. reticulata had the highest carbohydrate content (14.6 ± 0.4 g/100 mL, n = 3), and C. aurantifolia presented the highest protein content (34.1 ± 0.7 mg/100 mL). C. sinensis recorded the highest flavonoid content (91.4 ± 0.3 mg/100 mL), C. reticulata had the highest phenolic content (65.8 ± 0.6 mg/100 mL), and C. limon exhibited the highest ascorbic acid content (45.1 ± 0.4 mg/100 mL). The methanolic extracts of all citrus fruit juices demonstrated robust antioxidant properties, as determined by DPPH assay. Notably, C. limon and C. aurantifolia juice extracts demonstrated significant antimicrobial activity against a wide range of microbes. This study highlights the variation in nutrient and phytochemicals compositions among different citrus fruit juices, underscoring the nutritional and medicinal benefits of citrus species.http://www.sciencedirect.com/science/article/pii/S2405844024168041Citrus speciesJuiceNutraceutical propertiesPhytochemicalAntioxidant propertiesAntimicrobial activity
spellingShingle Nirmal Karki
Hari Achhami
Bishwa Bandhu Pachhai
Susmita Bhattarai
Dikpal Kumar Shahi
Lok Ranjan Bhatt
Mahesh Kumar Joshi
Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
Heliyon
Citrus species
Juice
Nutraceutical properties
Phytochemical
Antioxidant properties
Antimicrobial activity
title Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
title_full Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
title_fullStr Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
title_full_unstemmed Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
title_short Evaluating citrus juice: A comparative study of physicochemical, nutraceutical, antioxidant, and antimicrobial properties of citrus juices from Nepal
title_sort evaluating citrus juice a comparative study of physicochemical nutraceutical antioxidant and antimicrobial properties of citrus juices from nepal
topic Citrus species
Juice
Nutraceutical properties
Phytochemical
Antioxidant properties
Antimicrobial activity
url http://www.sciencedirect.com/science/article/pii/S2405844024168041
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