Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma
Molecular genetics abnormalities, underlying aberrant expression of E-cadherin and β-catenin in malignant tumors, are still unclear. Aim: to compare the mRNA expression levels of CDH1 and CTNNB1 genes and the immunohistochemical expression levels of E-cadherin and β-catenin proteins in intestinal...
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| Format: | Article |
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Zaporizhzhia State Medical and Pharmaceutical University
2017-04-01
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| Series: | Patologìâ |
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| Online Access: | http://pat.zsmu.edu.ua/article/view/97490/95636 |
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| author | V. A. Tumanskiy T. A. Khrystenko |
| author_facet | V. A. Tumanskiy T. A. Khrystenko |
| author_sort | V. A. Tumanskiy |
| collection | DOAJ |
| description | Molecular genetics abnormalities, underlying aberrant expression of E-cadherin and β-catenin in malignant tumors, are still unclear.
Aim: to compare the mRNA expression levels of CDH1 and CTNNB1 genes and the immunohistochemical expression levels of E-cadherin and β-catenin proteins in intestinal-type gastric adenocarcinoma.
Materials and methods. The molecular genetic (real-time PCR) and immunohistochemical studies of the intestinal-type gastric adenocarcinoma (30 patients; I, II and III clinical stages were included) and of the normal stomach mucosa (10 samples) were conducted.
Results. In comparison with the normal stomach mucosa, the intestinal-type gastric adenocarcinoma differs by the reduced transcriptional activity of the CDH1 gene [Me of relative normalized ratio of mRNA expression level is 0.14 (0.03; 0.40)], which correlates with the low expression level of E-cadherin in cancer cells [Me is 27,59 CUOD (23,14; 37,19), Spearman's correlation coefficient = 0,73], and also by the heightened transcriptional activity of the CTNNB1 gene [Me of relative normalized ratio of mRNA expression level is 4.32 (2.11; 11.43)], which correlates with the high expression level of β-catenin in cancer cells [Me is 116.65 CUOD (110.34; 151.32), Spearman's correlation coefficient = 0,94]. There is a reverse correlation between the CDH1 mRNA expression level and the degree of histological differentiation of intestinal-type gastric adenocarcinoma (correlation coefficient γ = -0,44); there is a direct correlation between the CTNNB1 and CDH1 mRNA expression levels (only for the I clinical stage cases); there are a direct correlations between the E-cadherin and β-catenin expression levels, and also between the CTNNB1 mRNA expression level and the E-cadherin expression level (only for the I-II clinical stage cases).
Conclusion. The data indicate that decrease of transcriptional activity of the CDH1 gene and E-cadherin expression level, and also increase of transcriptional activity of the CTNNB1 gene and β-catenin expression level in the cancer cells play a critical role in the process of progression of intestinal-type gastric adenocarcinoma.
gastric cancer; adenocarcinoma; CTNNB1 protein; human; CDH1 protein; human; beta Catenin; E-Cadherin |
| format | Article |
| id | doaj-art-8fbc982ae21d45b4ab1552cc9031d5ca |
| institution | DOAJ |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Patologìâ |
| spelling | doaj-art-8fbc982ae21d45b4ab1552cc9031d5ca2025-08-20T03:19:46ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372017-04-01141110.14739/2310-1237.2017.1.97490Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinomaV. A. TumanskiyT. A. KhrystenkoMolecular genetics abnormalities, underlying aberrant expression of E-cadherin and β-catenin in malignant tumors, are still unclear. Aim: to compare the mRNA expression levels of CDH1 and CTNNB1 genes and the immunohistochemical expression levels of E-cadherin and β-catenin proteins in intestinal-type gastric adenocarcinoma. Materials and methods. The molecular genetic (real-time PCR) and immunohistochemical studies of the intestinal-type gastric adenocarcinoma (30 patients; I, II and III clinical stages were included) and of the normal stomach mucosa (10 samples) were conducted. Results. In comparison with the normal stomach mucosa, the intestinal-type gastric adenocarcinoma differs by the reduced transcriptional activity of the CDH1 gene [Me of relative normalized ratio of mRNA expression level is 0.14 (0.03; 0.40)], which correlates with the low expression level of E-cadherin in cancer cells [Me is 27,59 CUOD (23,14; 37,19), Spearman's correlation coefficient = 0,73], and also by the heightened transcriptional activity of the CTNNB1 gene [Me of relative normalized ratio of mRNA expression level is 4.32 (2.11; 11.43)], which correlates with the high expression level of β-catenin in cancer cells [Me is 116.65 CUOD (110.34; 151.32), Spearman's correlation coefficient = 0,94]. There is a reverse correlation between the CDH1 mRNA expression level and the degree of histological differentiation of intestinal-type gastric adenocarcinoma (correlation coefficient γ = -0,44); there is a direct correlation between the CTNNB1 and CDH1 mRNA expression levels (only for the I clinical stage cases); there are a direct correlations between the E-cadherin and β-catenin expression levels, and also between the CTNNB1 mRNA expression level and the E-cadherin expression level (only for the I-II clinical stage cases). Conclusion. The data indicate that decrease of transcriptional activity of the CDH1 gene and E-cadherin expression level, and also increase of transcriptional activity of the CTNNB1 gene and β-catenin expression level in the cancer cells play a critical role in the process of progression of intestinal-type gastric adenocarcinoma. gastric cancer; adenocarcinoma; CTNNB1 protein; human; CDH1 protein; human; beta Catenin; E-Cadherinhttp://pat.zsmu.edu.ua/article/view/97490/95636gastric canceradenocarcinomaCTNNB1 proteinhumanCDH1 proteinbeta CateninE-Cadherin |
| spellingShingle | V. A. Tumanskiy T. A. Khrystenko Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma Patologìâ gastric cancer adenocarcinoma CTNNB1 protein human CDH1 protein beta Catenin E-Cadherin |
| title | Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma |
| title_full | Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma |
| title_fullStr | Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma |
| title_full_unstemmed | Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma |
| title_short | Comparative characteristics of the transcriptional activity of CDH1, CTNNB1 genes and the expression levels of E-cadherin, β-catenin proteins, coded by these genes, in intestinal-type gastric adenocarcinoma |
| title_sort | comparative characteristics of the transcriptional activity of cdh1 ctnnb1 genes and the expression levels of e cadherin β catenin proteins coded by these genes in intestinal type gastric adenocarcinoma |
| topic | gastric cancer adenocarcinoma CTNNB1 protein human CDH1 protein beta Catenin E-Cadherin |
| url | http://pat.zsmu.edu.ua/article/view/97490/95636 |
| work_keys_str_mv | AT vatumanskiy comparativecharacteristicsofthetranscriptionalactivityofcdh1ctnnb1genesandtheexpressionlevelsofecadherinbcateninproteinscodedbythesegenesinintestinaltypegastricadenocarcinoma AT takhrystenko comparativecharacteristicsofthetranscriptionalactivityofcdh1ctnnb1genesandtheexpressionlevelsofecadherinbcateninproteinscodedbythesegenesinintestinaltypegastricadenocarcinoma |