Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study
ObjectivesThis pilot study aimed to establish the interrelationship between collagen and mast cells in periapical granulomas and periapical cysts.Materials and MethodsAn observational cross-sectional study was conducted on the paraffin-embedded tissue sections of 68 specimens (34 periapical granulom...
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Korean Academy of Conservative Dentistry
2022-02-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://www.rde.ac/upload/pdf/rde-47-e12.pdf |
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| author | Deepty Bansal Mala Kamboj Anjali Narwal Anju Devi Nisha Marwah |
| author_facet | Deepty Bansal Mala Kamboj Anjali Narwal Anju Devi Nisha Marwah |
| author_sort | Deepty Bansal |
| collection | DOAJ |
| description | ObjectivesThis pilot study aimed to establish the interrelationship between collagen and mast cells in periapical granulomas and periapical cysts.Materials and MethodsAn observational cross-sectional study was conducted on the paraffin-embedded tissue sections of 68 specimens (34 periapical granulomas and 34 periapical cysts). The specimens were stained with picrosirius to observe collagen fiber birefringence and anti-tryptase antibody to evaluate the mast cell count immunohistochemically. The mean number and birefringence of collagen fibers, as well as the mean number of mast cells (total, granulated, and degranulated), and the mean inflammatory cell density were calculated. The data obtained were analyzed using the Kruskal Wallis test, Mann Whitney U test, and Spearman correlation test (p < 0.05).ResultsThe mean number of thick collagen fibers was higher in periapical cysts, while that of thin fibers was higher in granulomas (p = 0.00). Cysts emitted orange-yellow to red birefringence, whereas periapical granulomas had predominantly green fibers (p = 0.00). The mean inflammatory cell density was comparable in all groups (p = 0.129). The number of total, degranulated, and granulated mast cells exhibited significant results (p = 0.00) in both groups. Thick cyst fibers showed significant inverse correlations with inflammation and degranulated mast cells (p = 0.041, 0.04 respectively).ConclusionsMast cells and inflammatory cells influenced the nature of collagen fiber formation and its birefringence. This finding may assist in the prediction of the nature, pathogenesis, and biological behavior of periapical lesions. |
| format | Article |
| id | doaj-art-8fb4dc3c03f341659f89ad05dc652db0 |
| institution | DOAJ |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | Korean Academy of Conservative Dentistry |
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| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-8fb4dc3c03f341659f89ad05dc652db02025-08-20T02:42:15ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662022-02-01471e1210.5395/rde.2022.47.e121008Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical studyDeepty Bansal0Mala Kamboj1Anjali Narwal2Anju Devi3Nisha Marwah4Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pt B.D. Sharma University of Health Sciences, Rohtak, IndiaDepartment of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pt B.D. Sharma University of Health Sciences, Rohtak, IndiaDepartment of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pt B.D. Sharma University of Health Sciences, Rohtak, IndiaDepartment of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pt B.D. Sharma University of Health Sciences, Rohtak, IndiaDepartment of Pathology, Post Graduate Institute of Medical Sciences, Pt B.D. Sharma University of Health Sciences, Rohtak, IndiaObjectivesThis pilot study aimed to establish the interrelationship between collagen and mast cells in periapical granulomas and periapical cysts.Materials and MethodsAn observational cross-sectional study was conducted on the paraffin-embedded tissue sections of 68 specimens (34 periapical granulomas and 34 periapical cysts). The specimens were stained with picrosirius to observe collagen fiber birefringence and anti-tryptase antibody to evaluate the mast cell count immunohistochemically. The mean number and birefringence of collagen fibers, as well as the mean number of mast cells (total, granulated, and degranulated), and the mean inflammatory cell density were calculated. The data obtained were analyzed using the Kruskal Wallis test, Mann Whitney U test, and Spearman correlation test (p < 0.05).ResultsThe mean number of thick collagen fibers was higher in periapical cysts, while that of thin fibers was higher in granulomas (p = 0.00). Cysts emitted orange-yellow to red birefringence, whereas periapical granulomas had predominantly green fibers (p = 0.00). The mean inflammatory cell density was comparable in all groups (p = 0.129). The number of total, degranulated, and granulated mast cells exhibited significant results (p = 0.00) in both groups. Thick cyst fibers showed significant inverse correlations with inflammation and degranulated mast cells (p = 0.041, 0.04 respectively).ConclusionsMast cells and inflammatory cells influenced the nature of collagen fiber formation and its birefringence. This finding may assist in the prediction of the nature, pathogenesis, and biological behavior of periapical lesions.http://www.rde.ac/upload/pdf/rde-47-e12.pdfcollagen birefringencemast cellsperiapical cystperiapical granulomatryptase |
| spellingShingle | Deepty Bansal Mala Kamboj Anjali Narwal Anju Devi Nisha Marwah Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study Restorative Dentistry & Endodontics collagen birefringence mast cells periapical cyst periapical granuloma tryptase |
| title | Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study |
| title_full | Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study |
| title_fullStr | Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study |
| title_full_unstemmed | Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study |
| title_short | Interplay of collagen and mast cells in periapical granulomas and periapical cysts: a comparative polarizing microscopic and immunohistochemical study |
| title_sort | interplay of collagen and mast cells in periapical granulomas and periapical cysts a comparative polarizing microscopic and immunohistochemical study |
| topic | collagen birefringence mast cells periapical cyst periapical granuloma tryptase |
| url | http://www.rde.ac/upload/pdf/rde-47-e12.pdf |
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