Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure
Stress granules (SGs) are highly dynamic micromolecular membraneless condensates that generate in cells subjected to stress. Formed from pools of untranslating messenger ribonucleoproteins (RNP), SGs dynamics constitute vital processes essential for cell survival. Here, we investigate whether establ...
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Shared Science Publishers OG
2024-10-01
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| Series: | Cell Stress |
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| Online Access: | http://microbialcell.com/researcharticles/2024a-abdelrasol-cell-stress |
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| author | Hebatallah Abdelrasol Avika Chopra Liana Shvachiy Dirk Beutner Tiago F Outeiro Cristian Setz |
| author_facet | Hebatallah Abdelrasol Avika Chopra Liana Shvachiy Dirk Beutner Tiago F Outeiro Cristian Setz |
| author_sort | Hebatallah Abdelrasol |
| collection | DOAJ |
| description | Stress granules (SGs) are highly dynamic micromolecular membraneless condensates that generate in cells subjected to stress. Formed from pools of untranslating messenger ribonucleoproteins (RNP), SGs dynamics constitute vital processes essential for cell survival. Here, we investigate whether established cytotoxic agents, such as the platinum-based chemotherapeutic agent cisplatin and the aminoglycoside antibiotic gentamicin, elicit SG formation in the House Ear Institute-Organ of Corti-1 (HEI-OC1) auditory cell line, H4 human neuroglioma cells and HEK-293T human embryonic kidney cells. Cells were treated with cisplatin or gentamicin for specific durations at designated concentrations. SG formation was assessed using immunocytochemistry and live cell imaging. Levels of essential proteins involved in SG assembly were evaluated using immunoblotting. We observed cisplatin-associated SG assembly in HEI-OC1 and H4 cells via confocal microscopy through antibody colabeling of G3BP1 with PABP or Caprin1. While maintaining an unchanged pattern of expression of main constituent SG proteins, cisplatin-related SGs in H4 cells persisted for at least 12 h after drug removal. Cells subjected to gentamicin exposure did not exhibit SGs. Our findings offer insights into subcellular mechanisms related to cisplatin-associated cytotoxicity, highlighting the need for future studies to further investigate this stress-response mechanism. |
| format | Article |
| id | doaj-art-8fb2dfd83f1b40f1986b232ab07d4ce9 |
| institution | OA Journals |
| issn | 2523-0204 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Shared Science Publishers OG |
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| series | Cell Stress |
| spelling | doaj-art-8fb2dfd83f1b40f1986b232ab07d4ce92025-08-20T01:47:21ZengShared Science Publishers OGCell Stress2523-02042024-10-018839810.15698/cst2024.10.299Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposureHebatallah Abdelrasol0Avika Chopra1Liana Shvachiy2Dirk Beutner3Tiago F Outeiro4Cristian Setz5University Medical Center Göttingen, Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, Göttingen, GermanyUniversity Medical Center Göttingen, Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, Göttingen, GermanyUniversity Medical Center Göttingen, Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, Göttingen, GermanyUniversity Medical Center Göttingen, Department of Otolaryngology-Head and Neck Surgery, InnerEarLab, Göttingen, GermanyUniversity Medical Center Göttingen, Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, Göttingen, GermanyUniversity Medical Center Göttingen, Department of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, Göttingen, GermanyStress granules (SGs) are highly dynamic micromolecular membraneless condensates that generate in cells subjected to stress. Formed from pools of untranslating messenger ribonucleoproteins (RNP), SGs dynamics constitute vital processes essential for cell survival. Here, we investigate whether established cytotoxic agents, such as the platinum-based chemotherapeutic agent cisplatin and the aminoglycoside antibiotic gentamicin, elicit SG formation in the House Ear Institute-Organ of Corti-1 (HEI-OC1) auditory cell line, H4 human neuroglioma cells and HEK-293T human embryonic kidney cells. Cells were treated with cisplatin or gentamicin for specific durations at designated concentrations. SG formation was assessed using immunocytochemistry and live cell imaging. Levels of essential proteins involved in SG assembly were evaluated using immunoblotting. We observed cisplatin-associated SG assembly in HEI-OC1 and H4 cells via confocal microscopy through antibody colabeling of G3BP1 with PABP or Caprin1. While maintaining an unchanged pattern of expression of main constituent SG proteins, cisplatin-related SGs in H4 cells persisted for at least 12 h after drug removal. Cells subjected to gentamicin exposure did not exhibit SGs. Our findings offer insights into subcellular mechanisms related to cisplatin-associated cytotoxicity, highlighting the need for future studies to further investigate this stress-response mechanism.http://microbialcell.com/researcharticles/2024a-abdelrasol-cell-stresscisplatinh4 cellshearing losshei-oc1 cellsototoxicitystress granules |
| spellingShingle | Hebatallah Abdelrasol Avika Chopra Liana Shvachiy Dirk Beutner Tiago F Outeiro Cristian Setz Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure Cell Stress cisplatin h4 cells hearing loss hei-oc1 cells ototoxicity stress granules |
| title | Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure |
| title_full | Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure |
| title_fullStr | Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure |
| title_full_unstemmed | Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure |
| title_short | Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure |
| title_sort | stress granules formation in hei oc1 auditory cells and in h4 human neuroglioma cells secondary to cisplatin exposure |
| topic | cisplatin h4 cells hearing loss hei-oc1 cells ototoxicity stress granules |
| url | http://microbialcell.com/researcharticles/2024a-abdelrasol-cell-stress |
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