FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy
<b>Background</b>: Ovarian cancer (OC) is characterized by high incidence and mortality rates; however, due to its immunologically “cold” phenotype, the effectiveness of immunotherapy as a strategy for OC remains inadequate. Although the FAM111B gene promotes the progression of various s...
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MDPI AG
2025-05-01
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| author | Wanying Li Fang Wei Ting Zhou Lijuan Feng Lihong Zhang |
| author_facet | Wanying Li Fang Wei Ting Zhou Lijuan Feng Lihong Zhang |
| author_sort | Wanying Li |
| collection | DOAJ |
| description | <b>Background</b>: Ovarian cancer (OC) is characterized by high incidence and mortality rates; however, due to its immunologically “cold” phenotype, the effectiveness of immunotherapy as a strategy for OC remains inadequate. Although the FAM111B gene promotes the progression of various solid tumors, its specific function within the tumor immune microenvironment (TIME) of OC remains unclear. <b>Methods</b>: This study used multiplex immunofluorescence techniques and bioinformatics analysis to examine the role of FAM111B within the TIME of OC. Through multiplex immunofluorescence, we assessed the protein expression levels of FAM111B alongside key immune cell markers, including FOXP3, CD4, CD8, CD68, CD163, CD66b, and CD11c. Furthermore, we employed bioinformatics methods using The Cancer Genome Atlas database to validate FAM111B function at the mRNA level in OC. <b>Results</b>: We observed a positive correlation between FAM111B expression and immune cell infiltration, including T cells, macrophages, and dendritic cells. FAM111B, M2 macrophages, and regulatory T cells were associated with poorer overall survival in OC patients. Additionally, specific T cell subsets and dendritic cells were correlated positively with programmed death-ligand 1 expression, while FAM111B levels were linked to multiple immune checkpoint molecules. <b>Conclusions</b>: This study reveals a positive correlation between FAM111B overexpression and the infiltration levels of immune cells in OC. In OC patients characterized by elevated FAM111B expression, the potential augmentation of immune cell infiltration within the TIME may consequently enhance the efficacy of immunotherapy. |
| format | Article |
| id | doaj-art-8f9acf41899449bcaf3317a7ba8cbe0d |
| institution | OA Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-8f9acf41899449bcaf3317a7ba8cbe0d2025-08-20T02:24:35ZengMDPI AGBiomedicines2227-90592025-05-01136129510.3390/biomedicines13061295FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer ImmunotherapyWanying Li0Fang Wei1Ting Zhou2Lijuan Feng3Lihong Zhang4Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China<b>Background</b>: Ovarian cancer (OC) is characterized by high incidence and mortality rates; however, due to its immunologically “cold” phenotype, the effectiveness of immunotherapy as a strategy for OC remains inadequate. Although the FAM111B gene promotes the progression of various solid tumors, its specific function within the tumor immune microenvironment (TIME) of OC remains unclear. <b>Methods</b>: This study used multiplex immunofluorescence techniques and bioinformatics analysis to examine the role of FAM111B within the TIME of OC. Through multiplex immunofluorescence, we assessed the protein expression levels of FAM111B alongside key immune cell markers, including FOXP3, CD4, CD8, CD68, CD163, CD66b, and CD11c. Furthermore, we employed bioinformatics methods using The Cancer Genome Atlas database to validate FAM111B function at the mRNA level in OC. <b>Results</b>: We observed a positive correlation between FAM111B expression and immune cell infiltration, including T cells, macrophages, and dendritic cells. FAM111B, M2 macrophages, and regulatory T cells were associated with poorer overall survival in OC patients. Additionally, specific T cell subsets and dendritic cells were correlated positively with programmed death-ligand 1 expression, while FAM111B levels were linked to multiple immune checkpoint molecules. <b>Conclusions</b>: This study reveals a positive correlation between FAM111B overexpression and the infiltration levels of immune cells in OC. In OC patients characterized by elevated FAM111B expression, the potential augmentation of immune cell infiltration within the TIME may consequently enhance the efficacy of immunotherapy.https://www.mdpi.com/2227-9059/13/6/1295FAM111Bovarian cancerimmune cell infiltrationtumor immune microenvironmentimmunotherapy |
| spellingShingle | Wanying Li Fang Wei Ting Zhou Lijuan Feng Lihong Zhang FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy Biomedicines FAM111B ovarian cancer immune cell infiltration tumor immune microenvironment immunotherapy |
| title | FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy |
| title_full | FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy |
| title_fullStr | FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy |
| title_full_unstemmed | FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy |
| title_short | FAM111B Overexpression and Immune Cell Infiltration: Implications for Ovarian Cancer Immunotherapy |
| title_sort | fam111b overexpression and immune cell infiltration implications for ovarian cancer immunotherapy |
| topic | FAM111B ovarian cancer immune cell infiltration tumor immune microenvironment immunotherapy |
| url | https://www.mdpi.com/2227-9059/13/6/1295 |
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