The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L.
In this study, the functions of the photosynthetic machinery were evaluated using chlorophyll <i>a</i> fluorescence technique (PAM and JIP test) in pea plants (<i>Pisum sativum</i> L. cv Borec) and its LHC II oligomerization variants (mutants <i>Costata 2</i>/<...
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2025-06-01
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| author | Georgi D. Rashkov Martin A. Stefanov Amarendra N. Misra Emilia L. Apostolova |
| author_facet | Georgi D. Rashkov Martin A. Stefanov Amarendra N. Misra Emilia L. Apostolova |
| author_sort | Georgi D. Rashkov |
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| description | In this study, the functions of the photosynthetic machinery were evaluated using chlorophyll <i>a</i> fluorescence technique (PAM and JIP test) in pea plants (<i>Pisum sativum</i> L. cv Borec) and its LHC II oligomerization variants (mutants <i>Costata 2</i>/<i>133</i> and <i>Coeruleovireus 2</i>/<i>16</i>). The oligomeric forms of LHCII increased in the following order: <i>Costata 2</i>/<i>133</i> < Borec wt < <i>Coeruleovireus 2</i>/<i>16</i>. Data revealed that the mutant with higher LHCII oligomerization (<i>Coeruleovireus 2</i>/<i>16</i>) at low light intensity (LL, 150 µmol photons/m<sup>2</sup>·s) exhibited the following: (i) decreased energy dissipation and increased electron transport efficiency; (ii) higher reaction center density; (iii) increased amounts of the open reaction centers (qp) and their excitation efficiency (Φexc); and (iv) influenced the reoxidation of Q<sub>A<sup>−</sup></sub>, alleviating its interaction with plastoquinone. These effects enhanced photosynthetic performance related to PSII photochemistry (PI<sub>ABS</sub>) and overall photosynthetic efficiency (PItotal). High light intensity (HL, 500 µmol photons/m<sup>2</sup>·s) caused a reduction in open reaction centers (qp), excitation efficiency (Φexc), photochemical energy conversion of PSII (Φ<sub>PSII</sub>), maximum efficiency of PSII photochemistry in light (Fv′/Fm′), and linear electron transport via PSII, with more pronounced effects observed in membranes with a lower degree of LHCII oligomerization (<i>Costata 2</i>/<i>133</i>). This study provides novel experimental evidence for the pivotal role of the LHCII structural organization in determining the efficiency of light-dependent reactions of photosynthesis. |
| format | Article |
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| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-8f967ab4c5e643c2b50cedaa7a5f4ea52025-08-20T03:16:36ZengMDPI AGPlants2223-77472025-06-011412184610.3390/plants14121846The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L.Georgi D. Rashkov0Martin A. Stefanov1Amarendra N. Misra2Emilia L. Apostolova3Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaFaculty of Sciences, Sri Sri University, Cuttack 754006, Odisha, IndiaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaIn this study, the functions of the photosynthetic machinery were evaluated using chlorophyll <i>a</i> fluorescence technique (PAM and JIP test) in pea plants (<i>Pisum sativum</i> L. cv Borec) and its LHC II oligomerization variants (mutants <i>Costata 2</i>/<i>133</i> and <i>Coeruleovireus 2</i>/<i>16</i>). The oligomeric forms of LHCII increased in the following order: <i>Costata 2</i>/<i>133</i> < Borec wt < <i>Coeruleovireus 2</i>/<i>16</i>. Data revealed that the mutant with higher LHCII oligomerization (<i>Coeruleovireus 2</i>/<i>16</i>) at low light intensity (LL, 150 µmol photons/m<sup>2</sup>·s) exhibited the following: (i) decreased energy dissipation and increased electron transport efficiency; (ii) higher reaction center density; (iii) increased amounts of the open reaction centers (qp) and their excitation efficiency (Φexc); and (iv) influenced the reoxidation of Q<sub>A<sup>−</sup></sub>, alleviating its interaction with plastoquinone. These effects enhanced photosynthetic performance related to PSII photochemistry (PI<sub>ABS</sub>) and overall photosynthetic efficiency (PItotal). High light intensity (HL, 500 µmol photons/m<sup>2</sup>·s) caused a reduction in open reaction centers (qp), excitation efficiency (Φexc), photochemical energy conversion of PSII (Φ<sub>PSII</sub>), maximum efficiency of PSII photochemistry in light (Fv′/Fm′), and linear electron transport via PSII, with more pronounced effects observed in membranes with a lower degree of LHCII oligomerization (<i>Costata 2</i>/<i>133</i>). This study provides novel experimental evidence for the pivotal role of the LHCII structural organization in determining the efficiency of light-dependent reactions of photosynthesis.https://www.mdpi.com/2223-7747/14/12/1846chlorophyll <i>a</i> fluorescenceLHCIIpea mutantsphotosynthetic apparatus |
| spellingShingle | Georgi D. Rashkov Martin A. Stefanov Amarendra N. Misra Emilia L. Apostolova The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. Plants chlorophyll <i>a</i> fluorescence LHCII pea mutants photosynthetic apparatus |
| title | The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. |
| title_full | The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. |
| title_fullStr | The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. |
| title_full_unstemmed | The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. |
| title_short | The Role of Light-Harvesting Complex II Organization in the Efficiency of Light-Dependent Reactions in the Photosynthetic Apparatus of <i>Pisum sativum</i> L. |
| title_sort | role of light harvesting complex ii organization in the efficiency of light dependent reactions in the photosynthetic apparatus of i pisum sativum i l |
| topic | chlorophyll <i>a</i> fluorescence LHCII pea mutants photosynthetic apparatus |
| url | https://www.mdpi.com/2223-7747/14/12/1846 |
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