Transcriptomics and trans-organellar complementation reveal limited signaling of 12-cis-oxo-phytodienoic acid during early wound response in Arabidopsis
Abstract 12-cis-oxo-phytodienoic acid (OPDA), a precursor of jasmonoyl-isoleucine (JA-Ile), is known to have distinct signaling roles in Arabidopsis, as shown in studies using the opr3 mutant, which lacks OPDA REDUCTASE3 (OPR3). This mutant, however, accumulates low levels of JA-Ile through an OPR2-...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-61832-9 |
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| Summary: | Abstract 12-cis-oxo-phytodienoic acid (OPDA), a precursor of jasmonoyl-isoleucine (JA-Ile), is known to have distinct signaling roles in Arabidopsis, as shown in studies using the opr3 mutant, which lacks OPDA REDUCTASE3 (OPR3). This mutant, however, accumulates low levels of JA-Ile through an OPR2-mediated bypass. To investigate OPDA signaling, the wound-induced transcriptome of the opr2opr3 mutant is compared to that of wild-type and allene oxide synthase mutant. Endogenous OPDA shows no unique transcriptional signature under control or wounding conditions, and previously identified OPDA-responsive genes are wound-induced independently of OPDA. Applying OPDA to opr2opr3 triggers a distinct response suggesting compartmentalization of endogenously formed OPDA. Trans-organellar complementation reveals that expression of OPR3 or OPR2 in opr2opr3 restores JA-Ile production regardless of localization, whereas mitochondrial targeted OPR1 exhibiting low OPDA/4,5-ddh-JA conversion activity does not. Our findings show that OPDA primarily serves as a JA precursor with limited independent signaling functions in the early wound response. |
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| ISSN: | 2041-1723 |