Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients
Background and Objectives: Graft-versus-host disease (GvHD) frequently complicates hematopoietic stem cell transplantation (HSCT). Emerging evidence suggests a correlation between gut microbiota and GvHD risk. This study aims to elucidate the microbiota profiles in HSCT patients before and after tr...
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Tehran University of Medical Sciences
2024-10-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4497 |
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| author | Pegah Panahi Amir Hossein Hashemian Mehrdad Payandeh Mahdi Taghadosi Bizhan Nomanpour |
| author_facet | Pegah Panahi Amir Hossein Hashemian Mehrdad Payandeh Mahdi Taghadosi Bizhan Nomanpour |
| author_sort | Pegah Panahi |
| collection | DOAJ |
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Background and Objectives: Graft-versus-host disease (GvHD) frequently complicates hematopoietic stem cell transplantation (HSCT). Emerging evidence suggests a correlation between gut microbiota and GvHD risk. This study aims to elucidate the microbiota profiles in HSCT patients before and after transplantation and their association with GvHD.
Materials and Methods: This study, conducted from December 2022 to December 2023, involved the collection of 15 stool samples from HSCT patients. Bacterial content was quantified using real-time PCR, while interleukin-6 levels were assessed via ELISA.
Results: Among the 15 participants (8 male, 7 female), 9 underwent allogeneic HSCT (allo-HSCT) and 6 received autologous HSCT. In the aGvHD group, there was a significant reduction in the abundance of Bacteroides and Bifidobacterium compared to those without aGvHD. Additionally, declines were observed in Clostridium and Firmicutes populations. The genus Blautia also showed reduced prevalence in the aGvHD group, whereas no significant differences were noted in the uncomplicated group. ELISA analysis revealed that interleukin-6 levels remained within the normal range (30-960 pg/ml) with no significant elevation in the aGvHD group.
Conclusion: The study highlights a notable association between alterations in gut microbiota, specifically reductions in certain bacterial populations and the development of aGvHD following allo-HSCT.
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| format | Article |
| id | doaj-art-8f694425f248494c9d7d23599cce20b4 |
| institution | OA Journals |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-8f694425f248494c9d7d23599cce20b42025-08-20T02:14:02ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-10-0116510.18502/ijm.v16i5.16800Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patientsPegah Panahi0Amir Hossein Hashemian1Mehrdad Payandeh2Mahdi Taghadosi3Bizhan Nomanpour4Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Biostatistics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Bone Marrow Transplantation, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah IranDepartment of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Background and Objectives: Graft-versus-host disease (GvHD) frequently complicates hematopoietic stem cell transplantation (HSCT). Emerging evidence suggests a correlation between gut microbiota and GvHD risk. This study aims to elucidate the microbiota profiles in HSCT patients before and after transplantation and their association with GvHD. Materials and Methods: This study, conducted from December 2022 to December 2023, involved the collection of 15 stool samples from HSCT patients. Bacterial content was quantified using real-time PCR, while interleukin-6 levels were assessed via ELISA. Results: Among the 15 participants (8 male, 7 female), 9 underwent allogeneic HSCT (allo-HSCT) and 6 received autologous HSCT. In the aGvHD group, there was a significant reduction in the abundance of Bacteroides and Bifidobacterium compared to those without aGvHD. Additionally, declines were observed in Clostridium and Firmicutes populations. The genus Blautia also showed reduced prevalence in the aGvHD group, whereas no significant differences were noted in the uncomplicated group. ELISA analysis revealed that interleukin-6 levels remained within the normal range (30-960 pg/ml) with no significant elevation in the aGvHD group. Conclusion: The study highlights a notable association between alterations in gut microbiota, specifically reductions in certain bacterial populations and the development of aGvHD following allo-HSCT. https://ijm.tums.ac.ir/index.php/ijm/article/view/4497Gut microbiota;Hematopoietic stem cell transplantation;Graft-versus-host disease;Interleukin-6;Real-time polymerase chain reaction;Enzyme-linked immunosorbent assay |
| spellingShingle | Pegah Panahi Amir Hossein Hashemian Mehrdad Payandeh Mahdi Taghadosi Bizhan Nomanpour Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients Iranian Journal of Microbiology Gut microbiota; Hematopoietic stem cell transplantation; Graft-versus-host disease; Interleukin-6; Real-time polymerase chain reaction; Enzyme-linked immunosorbent assay |
| title | Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients |
| title_full | Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients |
| title_fullStr | Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients |
| title_full_unstemmed | Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients |
| title_short | Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients |
| title_sort | gut microbiota and graft versus host disease in hematopoietic stem cell transplant patients |
| topic | Gut microbiota; Hematopoietic stem cell transplantation; Graft-versus-host disease; Interleukin-6; Real-time polymerase chain reaction; Enzyme-linked immunosorbent assay |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4497 |
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