Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese

The aim of this study was to determine the influence of microbial (commercial starter) culture on concentration of total free amino groups (amino acids) in cheeses in different ripening stages. Free amino groups were determined by reaction with ninhydrin with cadmium (Cd) in the water soluble cheese...

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Main Authors: Biljana Radeljević, Nataša Mikulec, Neven Antunac, Zvonimir Prpić, Mirjana Maletić, Jasmina Havranek
Format: Article
Language:English
Published: Croatian Dairy Union 2013-03-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=144664
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author Biljana Radeljević
Nataša Mikulec
Neven Antunac
Zvonimir Prpić
Mirjana Maletić
Jasmina Havranek
author_facet Biljana Radeljević
Nataša Mikulec
Neven Antunac
Zvonimir Prpić
Mirjana Maletić
Jasmina Havranek
author_sort Biljana Radeljević
collection DOAJ
description The aim of this study was to determine the influence of microbial (commercial starter) culture on concentration of total free amino groups (amino acids) in cheeses in different ripening stages. Free amino groups were determined by reaction with ninhydrin with cadmium (Cd) in the water soluble cheese extract, and were expressed as the concentration of leucine in cheese dry matter. Changes in concentration of total free amino acids during cheese ripening (0th, 30th, 60th, 90th and 120th day) were monitored. In water soluble extracts of cheese, the presence of free NH2 groups in all ripening stages was detected, which means smaller peptides and amino acids, whose concentration significantly (P<0.01) increased during ripening. Cheeses produced with and without microbial culture resulted in statistically significant differences (P<0.01) in content amino acids free on the 90th and 120th day of ripening. Cd - ninhydrin method was found to be suitable for cheese ripening monitoring, as well as for determination of the differences in mature characteristics of cheeses, depending on the production process.
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1846-4025
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publishDate 2013-03-01
publisher Croatian Dairy Union
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series Mljekarstvo
spelling doaj-art-8f68556020ee425dbfc1dbe5813616fe2025-08-20T02:09:30ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252013-03-016311521Influence of starter culture on total free aminoacids concentration during ripening of Krk cheeseBiljana RadeljevićNataša MikulecNeven AntunacZvonimir PrpićMirjana MaletićJasmina HavranekThe aim of this study was to determine the influence of microbial (commercial starter) culture on concentration of total free amino groups (amino acids) in cheeses in different ripening stages. Free amino groups were determined by reaction with ninhydrin with cadmium (Cd) in the water soluble cheese extract, and were expressed as the concentration of leucine in cheese dry matter. Changes in concentration of total free amino acids during cheese ripening (0th, 30th, 60th, 90th and 120th day) were monitored. In water soluble extracts of cheese, the presence of free NH2 groups in all ripening stages was detected, which means smaller peptides and amino acids, whose concentration significantly (P<0.01) increased during ripening. Cheeses produced with and without microbial culture resulted in statistically significant differences (P<0.01) in content amino acids free on the 90th and 120th day of ripening. Cd - ninhydrin method was found to be suitable for cheese ripening monitoring, as well as for determination of the differences in mature characteristics of cheeses, depending on the production process.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=144664ewe cheese from the island of Krkcheese ripeningtotal free amino acidsmicrobial culture
spellingShingle Biljana Radeljević
Nataša Mikulec
Neven Antunac
Zvonimir Prpić
Mirjana Maletić
Jasmina Havranek
Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
Mljekarstvo
ewe cheese from the island of Krk
cheese ripening
total free amino acids
microbial culture
title Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
title_full Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
title_fullStr Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
title_full_unstemmed Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
title_short Influence of starter culture on total free aminoacids concentration during ripening of Krk cheese
title_sort influence of starter culture on total free aminoacids concentration during ripening of krk cheese
topic ewe cheese from the island of Krk
cheese ripening
total free amino acids
microbial culture
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=144664
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AT nevenantunac influenceofstartercultureontotalfreeaminoacidsconcentrationduringripeningofkrkcheese
AT zvonimirprpic influenceofstartercultureontotalfreeaminoacidsconcentrationduringripeningofkrkcheese
AT mirjanamaletic influenceofstartercultureontotalfreeaminoacidsconcentrationduringripeningofkrkcheese
AT jasminahavranek influenceofstartercultureontotalfreeaminoacidsconcentrationduringripeningofkrkcheese