Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis
Spirulina platensis contains several biologically active compounds, some of them with antioxidant activity. Nevertheless, not all of these compounds have been identified to date. As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioa...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/4605373 |
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| author | Margarita Cid-Hernández Fernando Antonio López Dellamary-Toral Luis Javier González-Ortiz María Judith Sánchez-Peña Fermín Paul Pacheco-Moisés |
| author_facet | Margarita Cid-Hernández Fernando Antonio López Dellamary-Toral Luis Javier González-Ortiz María Judith Sánchez-Peña Fermín Paul Pacheco-Moisés |
| author_sort | Margarita Cid-Hernández |
| collection | DOAJ |
| description | Spirulina platensis contains several biologically active compounds, some of them with antioxidant activity. Nevertheless, not all of these compounds have been identified to date. As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioautographies on silica gel thin layer chromatography plates was proposed. Starting with a reference binary system, 5 other binary systems were tested, in which the relative polarity was systematically increased. To further improve the separation behavior, a phase modifier (NH4OH) was used. The best separation results were obtained with the isopropyl alcohol/ethyl acetate/NH4OH ternary system. This experimental system allowed four well-resolved spots showing antioxidant activity as well as two additional areas with mixtures containing antioxidant compounds. Although the proposed methodology was designed with a specific application, it would be predictable that its field of use could be considerably greater, making the convenient modifications on the solvent polarity and “masking level” produced by the ammonium derivatives. |
| format | Article |
| id | doaj-art-8f535bde8f0b4db183e67f1005f3ff99 |
| institution | DOAJ |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-8f535bde8f0b4db183e67f1005f3ff992025-08-20T03:20:31ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/46053734605373Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensisMargarita Cid-Hernández0Fernando Antonio López Dellamary-Toral1Luis Javier González-Ortiz2María Judith Sánchez-Peña3Fermín Paul Pacheco-Moisés4Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, MexicoDepartamento de Madera, Celulosa y Papel, Universidad de Guadalajara, km 15.5 de la Carretera Guadalajara-Nogales, 45220 Zapopan, Jalisco, MexicoDepartamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, MexicoDepartamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, MexicoDepartamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, MexicoSpirulina platensis contains several biologically active compounds, some of them with antioxidant activity. Nevertheless, not all of these compounds have been identified to date. As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioautographies on silica gel thin layer chromatography plates was proposed. Starting with a reference binary system, 5 other binary systems were tested, in which the relative polarity was systematically increased. To further improve the separation behavior, a phase modifier (NH4OH) was used. The best separation results were obtained with the isopropyl alcohol/ethyl acetate/NH4OH ternary system. This experimental system allowed four well-resolved spots showing antioxidant activity as well as two additional areas with mixtures containing antioxidant compounds. Although the proposed methodology was designed with a specific application, it would be predictable that its field of use could be considerably greater, making the convenient modifications on the solvent polarity and “masking level” produced by the ammonium derivatives.http://dx.doi.org/10.1155/2018/4605373 |
| spellingShingle | Margarita Cid-Hernández Fernando Antonio López Dellamary-Toral Luis Javier González-Ortiz María Judith Sánchez-Peña Fermín Paul Pacheco-Moisés Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis International Journal of Analytical Chemistry |
| title | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis |
| title_full | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis |
| title_fullStr | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis |
| title_full_unstemmed | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis |
| title_short | Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis |
| title_sort | two dimensional thin layer chromatography bioautography designed to separate and locate metabolites with antioxidant activity contained on spirulina platensis |
| url | http://dx.doi.org/10.1155/2018/4605373 |
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