The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model
Prior to modern typing methods, cross-infection of P. aeruginosa between people with cystic fibrosis (CF) was felt to be rare. Recently a number of studies have demonstrated the presence of clonal strains of P. aeruginosa infecting people with CF. The aim of this study was to determine whether strai...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2011/712618 |
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| author | I. J. Clifton M. Denton F. M'Zali D. G. Peckham |
| author_facet | I. J. Clifton M. Denton F. M'Zali D. G. Peckham |
| author_sort | I. J. Clifton |
| collection | DOAJ |
| description | Prior to modern typing methods, cross-infection of P. aeruginosa between people with cystic fibrosis (CF) was felt to be rare. Recently a number of studies have demonstrated the presence of clonal strains of P. aeruginosa infecting people with CF. The aim of this study was to determine whether strains of P. aeruginosa demonstrated differences in resistance to desiccation and whether preincubation in subminimum inhibitory concentrations (MICs) of β-lactam affected desiccation resistance. The experimental data were modelled to a first-order decay model and a Weibull decay model using least squares nonlinear regression. The Weibull model was the preferred model for the desiccation survival. The presence of a mucoid phenotype promoted desiccation survival. Preincubation with antibiotics did not have a consistent effect on the strains of P. aeruginosa. Meropenem reduced desiccation resistance, whereas ceftazidime had much less effect on the strains studied. |
| format | Article |
| id | doaj-art-8f4b7a75eb054f69a8664e6b93b44147 |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-8f4b7a75eb054f69a8664e6b93b441472025-08-20T02:09:21ZengWileyInternational Journal of Microbiology1687-918X1687-91982011-01-01201110.1155/2011/712618712618The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival ModelI. J. Clifton0M. Denton1F. M'Zali2D. G. Peckham3Regional Adult Cystic Fibrosis Unit, St James University Hospital, Leeds LS9 7TF, UKDepartment of Microbiology, Leeds General Infirmary, Leeds LS1 3EX, UKDepartment of Microbiology, Leeds General Infirmary, Leeds LS1 3EX, UKRegional Adult Cystic Fibrosis Unit, St James University Hospital, Leeds LS9 7TF, UKPrior to modern typing methods, cross-infection of P. aeruginosa between people with cystic fibrosis (CF) was felt to be rare. Recently a number of studies have demonstrated the presence of clonal strains of P. aeruginosa infecting people with CF. The aim of this study was to determine whether strains of P. aeruginosa demonstrated differences in resistance to desiccation and whether preincubation in subminimum inhibitory concentrations (MICs) of β-lactam affected desiccation resistance. The experimental data were modelled to a first-order decay model and a Weibull decay model using least squares nonlinear regression. The Weibull model was the preferred model for the desiccation survival. The presence of a mucoid phenotype promoted desiccation survival. Preincubation with antibiotics did not have a consistent effect on the strains of P. aeruginosa. Meropenem reduced desiccation resistance, whereas ceftazidime had much less effect on the strains studied.http://dx.doi.org/10.1155/2011/712618 |
| spellingShingle | I. J. Clifton M. Denton F. M'Zali D. G. Peckham The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model International Journal of Microbiology |
| title | The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model |
| title_full | The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model |
| title_fullStr | The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model |
| title_full_unstemmed | The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model |
| title_short | The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model |
| title_sort | effect of sub mic β lactam antibiotic exposure of pseudomonas aeruginosa strains from people with cystic fibrosis in a desiccation survival model |
| url | http://dx.doi.org/10.1155/2011/712618 |
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