From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research
The mass rearing of anopheline mosquitoes under laboratory conditions is essential for advancing malaria research. It facilitates in-depth studies on mosquito biology, behavior, and genetics and their role in Plasmodium transmission. However, the colonization of Neotropical anophelines such as Anoph...
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Bio-protocol LLC
2025-05-01
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| author | Maisa Araujo Alice Andrade Alessandra Bastos Najara Santos José Pontual Jéssica Araújo Marina Rocha Maria Miguel Ana Costa Joseph Vinetz Ricardo Gazzinelli Jansen Medeiros |
| author_facet | Maisa Araujo Alice Andrade Alessandra Bastos Najara Santos José Pontual Jéssica Araújo Marina Rocha Maria Miguel Ana Costa Joseph Vinetz Ricardo Gazzinelli Jansen Medeiros |
| author_sort | Maisa Araujo |
| collection | DOAJ |
| description | The mass rearing of anopheline mosquitoes under laboratory conditions is essential for advancing malaria research. It facilitates in-depth studies on mosquito biology, behavior, and genetics and their role in Plasmodium transmission. However, the colonization of Neotropical anophelines such as Anopheles darlingi—a primary malaria vector in the Amazon region—has proven particularly challenging due to its unique reproductive characteristics. Unlike other species that can initially be colonized using forced copulation methods and later adapt to natural mating, An. darlingi does not copulate under forced conditions. Recent breakthroughs in An. darlingi colonization have been achieved using flashlight induction techniques, which have enabled the establishment and maintenance of stable laboratory populations. These advancements have created new opportunities for vector control studies in Brazil, including the testing of innovative control methods and Plasmodium transmission-blocking strategies. This protocol offers a comprehensive, step-by-step guide for initiating and scaling up large laboratory colonies of An. darlingi and An. deaneorum, a secondary malaria vector. It details methods for copulation induction, colony management, and successful artificial infection of mosquitoes with Plasmodium vivax. The guide serves as a critical resource for establishing new Neotropical anopheline colonies from different populations, contributing to future malaria research and control efforts in the Amazon. Additionally, the establishment of Brazil’s first Malaria Vector Production and Infection Platform (Plataforma de Produção e Infecção de Vetores da Malária, PIVEM) has further supported the development of new control technologies and the study of P. vivax–Anopheles interaction, advancing efforts to combat malaria in the region. |
| format | Article |
| id | doaj-art-8f37407b3f3147c5a4cc800df170ea30 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-8f37407b3f3147c5a4cc800df170ea302025-08-20T02:14:01ZengBio-protocol LLCBio-Protocol2331-83252025-05-0115910.21769/BioProtoc.5302From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria ResearchMaisa Araujo0Alice Andrade1Alessandra Bastos2Najara Santos3José Pontual4Jéssica Araújo5Marina Rocha6Maria Miguel7Ana Costa8Joseph Vinetz9Ricardo Gazzinelli10Jansen Medeiros11Plataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilPrograma de Pós-Graduação em Conservação e uso de Recursos Naturais – PPGReN, Fundação Universidade Federal de Rondônia, Porto Velho, Rondonia, Brazil, Laboratório de Pesquisa Translacional e Clínica, Centro de Pesquisa em Medicina Tropical, Porto Plataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilPrograma de Pós-Graduação em Saúde Pública, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, BrasilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilInstituto Nacional de Epidemiologia da Amazônia Ocidental - INCT-EpI-AmO, Porto Velho, RO, BrasilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilInstituto Nacional de Epidemiologia da Amazônia Ocidental - INCT-EpI-AmO, Porto Velho, RO, BrasilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilInstituto Nacional de Epidemiologia da Amazônia Ocidental - INCT-EpI-AmO, Porto Velho, RO, BrasilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilPrograma de Pós-Graduação em Conservação e uso de Recursos Naturais – PPGReN, Fundação Universidade Federal de Rondônia, Porto Velho, Rondonia, BrazilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilInstituto Nacional de Epidemiologia da Amazônia Ocidental - INCT-EpI-AmO, Porto Velho, RO, BrasilSection of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USALaboratório de Imunopatologia, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, BrazilDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USAPlataforma de Produção e Infecção de Vetores da Malária (PIVEM), Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Rondonia, BrazilInstituto Nacional de Epidemiologia da Amazônia Ocidental - INCT-EpI-AmO, Porto Velho, RO, BrasilThe mass rearing of anopheline mosquitoes under laboratory conditions is essential for advancing malaria research. It facilitates in-depth studies on mosquito biology, behavior, and genetics and their role in Plasmodium transmission. However, the colonization of Neotropical anophelines such as Anopheles darlingi—a primary malaria vector in the Amazon region—has proven particularly challenging due to its unique reproductive characteristics. Unlike other species that can initially be colonized using forced copulation methods and later adapt to natural mating, An. darlingi does not copulate under forced conditions. Recent breakthroughs in An. darlingi colonization have been achieved using flashlight induction techniques, which have enabled the establishment and maintenance of stable laboratory populations. These advancements have created new opportunities for vector control studies in Brazil, including the testing of innovative control methods and Plasmodium transmission-blocking strategies. This protocol offers a comprehensive, step-by-step guide for initiating and scaling up large laboratory colonies of An. darlingi and An. deaneorum, a secondary malaria vector. It details methods for copulation induction, colony management, and successful artificial infection of mosquitoes with Plasmodium vivax. The guide serves as a critical resource for establishing new Neotropical anopheline colonies from different populations, contributing to future malaria research and control efforts in the Amazon. Additionally, the establishment of Brazil’s first Malaria Vector Production and Infection Platform (Plataforma de Produção e Infecção de Vetores da Malária, PIVEM) has further supported the development of new control technologies and the study of P. vivax–Anopheles interaction, advancing efforts to combat malaria in the region.https://bio-protocol.org/en/bpdetail?id=5302&type=0 |
| spellingShingle | Maisa Araujo Alice Andrade Alessandra Bastos Najara Santos José Pontual Jéssica Araújo Marina Rocha Maria Miguel Ana Costa Joseph Vinetz Ricardo Gazzinelli Jansen Medeiros From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research Bio-Protocol |
| title | From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research |
| title_full | From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research |
| title_fullStr | From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research |
| title_full_unstemmed | From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research |
| title_short | From Colonization to High Production and Plasmodium vivax Infection of Anopheles darlingi and Anopheles deaneorum: a Platform for Malaria Research |
| title_sort | from colonization to high production and plasmodium vivax infection of anopheles darlingi and anopheles deaneorum a platform for malaria research |
| url | https://bio-protocol.org/en/bpdetail?id=5302&type=0 |
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